摘要
目的:制备替硝唑外用凝胶;并以甲硝唑为内标,采用高效液相色谱法测定替硝唑凝胶剂的含量。方法:采用Hyper-sil-C_(18)柱(5μm,5mm×250mm);以甲醇-水-36％醋酸(30:70:0.5)为流动相;流速为1.0mL·min^(-1);检测波长为318nm;灵敏度为0.08 AUFS。结果:替硝唑浓度在41.84～125.52mg·L^(-1)范围内呈良好的线性关系,r=0.9997;平均回收率为97.92％,RSD为1.02％。结论:该方法快速,准确,重现性好,适用于替硝唑凝胶剂的质量控制。
OBJECTIVE To prepare a tinidazole gel and establish an HPLC method with metronidazole as internal standard to determine the content. METHODS The chromatographic column; Hypersil-C18 column. Mobile phase:methanol-water-36%acetic acid (30:70:0.5). Flow rate: 1.0 mL·min-1. Detection wavelength: 318 nm. Sensitivity: 0.08 AUFS. RESULTS The calibration curve was linear in the range of 41.84-125.52 mg·L-1 ( r = 0. 9997). Average recovery was 97. 92 % with RSD 1.02%.CONCLUSIONS The method is accurate, rapid, and suitable for the quality-control of the gel.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第12期739-740,共2页
Chinese Journal of Hospital Pharmacy
