摘要
目的：应用双波长分光光度法测定替硝唑、环丙沙星注射液含量。方法：以ｐＨ４．５的醋酸盐缓冲液为溶剂，在波长３１７，２９７．６，２７７，３５４．２ｎｍ处进行测定。。结果：替硝唑浓度在４～２０μｇ·ｍｌ－１之间，环丙沙星浓度在２～１０μｇ·ｍｌ－１之间符合比尔定律，浓度与吸收度差值呈良好的线性关系。替硝唑平均回收率为１００．８％，ＲＳＤ为１．０％（ｎ＝９），环丙沙星平均回收率为９８．８％，ＲＳＤ为０．８％（ｎ＝９）。结论：该方法简便、快速、准确可靠。
OBJECTIVE :The tinidazole and ciprofloxacin injection content was determined by dual wavelength spectrophotometry. METHODSTBZ:It was determined by using acetate buffer (pH4.5) as the solvent at the wavelength of 317 nm,297.6 nm,277 nm,and 354.2 nm. RESULTSZ:The results showed that both the standard curve of tinidazole concentration ranging from 4 to 20 gml-1 and ciprofloxacin concentration ranging from 2 to 10 gml-1 was in correspondence with Beer's law.The absorptivity difference and the concentration showed a good linear relationship.The mean recovery of tinidazole was 100.8% with RSD 1.0% (n=9),while the ciprofloxacin was 98.8% with RSD 0.8% (n=9). CONCLUSIONSBZ:This method is simple,quick and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第5期283-285,共3页
Chinese Journal of Hospital Pharmacy
关键词
双波长
分光光度法
替硝唑
环丙沙星
注射液
dual wavelength spectrophotometry,tinidazole,ciprofloxacin injection
