摘要
目的 研制乙酰胆碱酯酶 (Acetylcholinesterase ,AchE)抗独特型抗体并测定酶活性。方法 AchE单克隆抗体是一种IgG1抗体 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA亲和层析柱进行提取纯化 ,获得纯化的单克隆抗体Fab段。以Fab作为抗原 ,免疫小鼠 ,制备抗Fab独特型抗体 ,采用酶催化ELISA法研究该单克隆抗体的乙酰胆碱酯酶活性。结果 制备出高效价抗乙酰胆碱酯酶抗体Fab段的独特型抗体 ,该抗体具有乙酰胆碱酯酶活性。结论 AchE单克隆抗体的独特型抗体具有AchE活性。
ObjectiveTo study the enzyme activity of antibody on a nt i acetylcholinesterase antibody.MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. Anti Fab monoclonal antibody was prepared with hyb ridoma. Results Anti Fab idiotype antibody was prepared. Enzy me activity of idiotype antibody was detected with ELISA.Conclusion 3F3 Fab idiotype has been prepared,it has been proved that it has AchE acti vity.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第1期30-32,共3页
Immunological Journal
基金
山东省自然科学基金项目 (9715 0 5 1)
关键词
乙酰胆碱酯酶
单克隆抗体
抗独特型抗体
抗体酶
Acetylcholinesterase
Monoclonal antibody
Anti idiotype antibody
Ab Enzyme
