摘要
【目的】功能型NAM-B1基因在成熟过程中,促进营养物质从茎、叶等部位向籽粒转移分配,是培育籽粒高蛋白、高微量元素(Fe、Zn)和早熟小麦品种的重要靶基因。然而,功能型NAM-B1基因是否在我国小麦育种中得到了应用,还不清楚。【方法】以早熟高产小麦新品种蜀麦1963为材料,通过克隆测序和籽粒灌浆速率测定,分析其是否携带了功能型NAM-B1基因,并且分析其与籽粒灌浆速率之间的关系。【结果】蜀麦1963携带功能型NAM-B1基因,且能显著提高籽粒灌浆速率。同时,蜀麦1963的NAM-A1和NAM-D1位点也为功能型等位类型,分别为NAM-A1a和NAM-D1 Hap1。【结论】3个部分同源位点均携带功能型NAM-1基因,可能是蜀麦1963灌浆速率高、灌浆持续时间缩短,进而实现早熟的重要遗传基础。功能型NAM-B1基因可以用于早熟高产小麦品种的培育。蜀麦1963是利用NAM-1基因进行早熟高产小麦培育的育种亲本。
【Objective】Functional NAM-B1 gene promotes nutrient remobilisation from stem and leaves to grains during senescence process,and is an important target gene for developing high protein,high iron(Fe)and zinc(Zn),and early maturing wheat varieties.However,whether the functional NAM-B1 gene has been included into Chinese varieties is still unknown.【Method】The NAM-B1 gene and its relation with grain-filling duration in the early maturing and high-yielding wheat variety Shumai 1963 was characterized through gene sequencing and grain filling rate determination.【Result】Shumai 1963 carries the functional NAM-B1 gene,which was associated with a high grain-filling rate.Meanwhile,both the NAM-A1 and NAM-D1 loci in Shumai 1963 are also functional allele types,namely NAM-A1a and NAM-D1 Hap1,respectively.【Conclusion】Carrying three functional NAM-1 homologous loci may be an important genetic basis for the high grain filling rate and shortened grain filling duration of Shumai 1963,thus achieving early maturity.The functional NAM-B1 gene can be used for the developing of early maturing and high-yielding wheat varieties.Shumai 1963 is a bridge parent for early maturing and high-yielding wheat breeding via the NAM-1 gene.
作者
李生科
祝婷婷
王佳恒
陈雪
陈雪姣
范超兰
姜博
黄林
甯顺腙
袁中伟
张连全
杨洪坤
江千涛
樊高琼
张一靖
毛龙
刘登才
郝明
LI Shengke;ZHU Tingting;WANG Jiaheng;CHEN Xue;CHEN Xuejiao;FAN Chaolan;JIANG Bo;HUANG Lin;NING Shunzong;YUAN Zhongwei;ZHANG Lianquan;YANG Hongkun;JIANG QianTao;FAN Gaoqiong;ZHANG Yijing;MAO Long;LIU Dengcai;HAO Ming(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,China;College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China;State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Chengdu 611130,China;School of Life Sciences,Fudan University,Shanghai 200438,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《四川农业大学学报》
北大核心
2026年第1期1-7,共7页
Journal of Sichuan Agricultural University
基金
国家自然科学基金(U24A20401)
四川省科技计划(2021YFYZ0002、2025YFHZ0113)
四川省麦类产业技术体系(SCCXTD-2024-11)。
