摘要
以加工中废弃柿皮为研究对象,采用单因素结合响应面法优化柿皮总黄酮的超声辅助乙醇提取工艺,并评价优化工艺下提取的柿皮黄酮对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基和羟自由基的清除效果,以及铁离子还原能力(Ferric ion reducing antioxidant power,FRAP)。结果表明,优化后最佳提取工艺条件为乙醇体积分数81.5%、液料比24.6:1 mL/g、提取温度53℃、提取时间45 min,柿皮黄酮提取量最高为(10.55±0.03)mg/g。抗氧化活性评价结果显示,柿皮黄酮对DPPH自由基、ABTS阳离子自由基和羟自由基清除的IC_(50)值分别为<0.1、0.43 mg/mL和0.297 mg/mL,在0.1~0.4 mg/mL浓度范围内,柿皮黄酮FRAP优于维生素C(VC),表明柿皮黄酮具有较好的抗氧化活性。本研究为废弃柿皮功能组分开发提供了参考。
The ultrasound-assisted ethanol extraction process for total flavonoids from persimmon peel was systematically optimized using a combination of single-factor experiments and response surface methodology(RSM).Subsequent antioxidant activity assessments of flavonoids extracted were carried out,under optimized conditions included:scavenging effects on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals,2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radicals,and hydroxyl radicals;and ferric ion reducing antioxidant power(FRAP)analysis.RSM optimization revealed the following ideal parameters:ethanol concentration 81.5%,liquid-to-solid ratio 24.6:1 mL/g,extraction temperature 53 C,and duration 45 min.Under these conditions,the maximum flavonoid yield reached(10.55±0.03)mg/g dry weight.The results of antioxidant activity evaluation showed that the IC_(50)values of persimmon peel flavonoids on DPPH radicals,ABTS cation radical and hydroxyl radical scavenging were<0.1 mg/mL,0.43 mg/mL and 0.297 mg/mL,respectively.Within 0.1-0.4 mg/mL range,the FRAP surpassed that of ascorbic acid(VC)controls.These findings substantiate the potent antioxidant activity of persimmon peel flavonoids,providing a scientific basis for valorizing this agricultural byproduct in functional ingredient development.
作者
张献忠
魏解冰
李阳
乔西磊
安子豪
马自强
ZHANG Xianzhong;WEI Jiebing;LI Yang;QIAO Xilei;AN Zihao;MA Ziqiang(College of Life Science and Engineering,Handan University,Handan 056005,China)
出处
《食品科技》
北大核心
2025年第12期226-234,共9页
Food Science and Technology
基金
河北省高等学校科学研究项目(ZC2025036)
邯郸学院校级项目(XZ2024025,XZ2022047)。
关键词
柿皮
提取工艺
总黄酮
抗氧化
persimmon peel
extraction technology
total flavonoids
antioxidant activity
