摘要
目的建立一种操作简便快速、线性范围宽、准确灵敏的游泳池水中尿素测定方法。方法按照《公共场所卫生检验方法第2部分:化学污染物》(GB/T 18204.2—2014)(简称国标法),通过对水浴时间、试剂种类和浓度、标准系列等进行改进,用分光光度计测定游泳池水中尿素含量,进行方法学评价。结果改进后的国标法(简称改进法)质量浓度范围为0~3.50 mg/L,相关系数(r)=0.9998,相对标准偏差(RSD,n=6)为1.17%~4.75%,加标回收率为97.61%~103.06%,方法检出限为0.082 mg/L。改进法和国标法测定43份游泳池水样中尿素含量检测的结果差异无统计学意义(Z=-0.013,P=0.990)。结论改进法精密度和准确度高,线性范围宽,加热时间短,显色产物稳定,适用于游泳池水中尿素的定量检测。
Objective To establish a simple,fast,wide linear range,accurate and sensitive method for the determination of urea in swimming pool water.Methods According to Examination Methods for Public Places-Part 2:Chemical Pollutants(GB/T 18204.2-2014)(hereinafter referred to as the national standard method),the water bath time,types and concentrations of reagents,standard series,etc.were optimized.The urea content in swimming pool water was measured by using spectrophotometer for methodological evaluation.Results The concentration range of improved national standard method(referred to as the improved method)was 0-3.50 mg/L,the correlation coefficient was 0.9998,the relative standard deviation(RSD,n=6)was 1.17%-4.75%,the recovery rate was 97.61%-103.06%,and the detection limit of the method was 0.082 mg/L.There was no statistically significant difference between the improved method and the national standard method for measuring the urea content in 43 swimming pool water samples(Z=‒0.013,P=0.990).Conclusion Improved method has high precision and accuracy,wide linear range,short heating time,stable color products,and is suitable for quantitative detection of urea in swimming pool water.
作者
姚远
李香玉
徐悦桄
YAO Yuan;LI Xiangyu;XU Yueguang(Yunlong District Center for Disease Control and Prevention,Xuzhou 221000,Jiangsu,China)
出处
《安徽预防医学杂志》
2025年第6期519-523,共5页
Anhui Journal of Preventive Medicine
关键词
游泳池水
尿素
二乙酰-肟法
氯化铁
Swimming pool water
Urea
Diacetyl oxime method
Ferric chloride
