摘要
斑马鱼凭借其胚胎透明性、遗传可操作性及与哺乳动物高度保守的肝脏结构与功能,已成为肝脏疾病机制研究与药物开发的重要模式生物。通过系统综述斑马鱼在肝损伤、肝纤维化及肝细胞癌中的模型构建方法,重点探讨中药组分在改善肝损伤、抗纤维化及抗肿瘤方面的治疗作用,旨在为拓展斑马鱼模型在肝脏疾病研究中的应用及推动中药防治肝脏疾病的研究提供新思路与方法。
The scale-up of traditional Chinese medicine(TCM)extraction processes is a crucial step in transitioning from laboratory research to industrial production.With the rapid development of the TCM industry,the scale-up of TCM extraction processes faces numerous key challenges,such as parameter fluctuations caused by multi-factor coupling,instability in cross-scale amplification due to unclear kinetic mechanisms,and quality variations resulting from the failure of traditional scaling-up theories.In recent years,with the rapid advancement of process analytical technology(PAT),its application in the scale-up of TCM extraction processes has garnered increasing attention.PAT enhances the efficiency and stability of TCM extraction process scale-up by real-time monitoring and analyzing key parameters during the extraction process,optimizing extraction techniques,establishing precise models,and implementing dynamic regulation and feedback control.This provides robust data support for process scale-up.This review summarizes the application of PAT in extraction process scale-up,systematically elaborates on the current research status of data acquisition,process modeling,and dynamic feedback techniques,and analyzes the current problems and challenges faced by extraction process scale-up.It aims to provide insights for promoting the application of PAT in the TCM manufacturing industry,thereby facilitating the intelligent transformation,upgrading,and high-quality development of the TCM industry.
作者
管咏梅
刘乐灵
杨明
郑琴
伍振峰
王丹凤
吴样明
钟光德
臧振中
GUAN Yongmei;LIU Leling;YANG Ming;ZHENG Qin;WU Zhenfeng;WANG Danfeng;WU Yangming;ZHONG Guangde;ZANG Zhenzhong(Key Laboratory of Modern Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of ChineseMedicine,Nanchang 330004,China;State Key Laboratory for the Modernization of Classical and Famous Prescriptions of Chinese Medicine,Nanchang 330004,China;Sichuan Association for Quality Management of Pharmaceutical,Health and Cosmetics Products,Chengdu 610000,China)
出处
《中草药》
北大核心
2026年第2期749-766,共18页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(82460841)
甘肃省重点人才(青年团队)项目(2024QNTD36)
甘肃省自然科学基金项目(22JR11RA114)
甘肃省高校产业支撑计划项目(2024CYZC-39)
甘肃省中医药研究中心开放课题(zyzx-2023-01)
甘肃中医药大学校级创新创业项目(2026CXCY-319)。
关键词
斑马鱼
肝损伤
肝纤维化
肝细胞癌
中药
process analytical technology
extraction process scale-up
intelligent regulation and control
data acquisition
process modeling
dynamic feedback techniques
