摘要
目的:建立能同时测定菟芪合剂中芍药苷、松脂醇二葡萄糖苷、毛蕊异黄酮葡萄糖苷、甘草苷、金丝桃苷、川续断皂苷Ⅵ含量的一测多评定量分析方法。方法:采用高效液相色谱法,利用Thermo Hypersil C_(18)色谱柱(250.0 mm×4.6 mm,5.0μm);流动相为0.1%磷酸溶液-甲醇,梯度洗脱;流速为1.0 mL/min;柱温为30℃;DAD检测器。以甘草苷为内参物,分别构建其余5种成分的相对校正因子,进而计算含量。结果:芍药苷、松脂醇二葡萄糖苷、毛蕊异黄酮葡萄糖苷、金丝桃苷、川续断皂苷Ⅵ的相对校正因子分别为2.037 0、0.407 9、0.512 7、0.827 8、9.656 1;12批菟芪合剂中6个成分的一测多评法所得结果与外标法的测定值无显著差别。结论:该方法高效快速,结果准确、可靠,可用于菟芪合剂中6种成分的同时测定,为菟芪合剂的质量控制及标准建立提供了依据。
Objective:To establish a method for the simultaneous determination of paeoniflorin,pinoresinol diglucoside,calycosin-7-O-β-D-glucoside,liquiritin,hyperoside,and asperosaponinⅥin Tuqi mixture using quantitative analysis of multi-components by single marker(QAMS).Methods:A high-performance liquid chromatography(HPLC)method was employed.The separation was performed on a Thermo Hypersil C_(18) column(250.0 mm×4.6 mm,5.0μm).The mobile phase consisted of methanol and 0.1%phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min.The column temperature was maintained at 30°C,and detection was carried out using a DAD detector.With liquiritin as the internal reference substance,the relative correction factors(RCFs)of the other five components were calculated to determine their contents.Results:The RCFs for paeoniflorin,pinoresinol diglucoside,calycosin-7-O-β-D-glucoside,hyperoside,and asperosaponinⅥwere 2.0370,0.4079,0.5127,0.8278,and 9.6561,respectively.For 12 batches of Tuqi mixture,the results obtained by the QAMS method for the six components showed no significant difference compared with those determined by the external standard method.Conclusion:The established method is efficient,rapid,accurate,and reliable,and it can be used for the simultaneous determination of the six components in Tuqi mixture,providing a basis for the quality control and standard establishment of Tuqi mixture.
作者
杨扬宇
谢谦
邱盼子
肖作奇
欧阳波
YANG Yangyu;XIE Qian;QIU Panzi;XIAO Zuoqi;OUYANG Bo(Hunan Provincial Maternal and Child Health Care Hospital,Changsha Hunan 410008,China;Hunan Engineering Research Center of Traditional Chinese Medicine Clinical Formula Transformation for Women and Children,Changsha Hunan 410008,China)
出处
《中医药导报》
2026年第1期87-91,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
长沙市自然科学基金项目(kq2502019)
长沙市科技创新平台项目(kh2005013)。
