摘要
目的探讨醋酸去氧皮质酮(DOCA)诱导高血压大鼠模型的稳定性,并比较手术组(单侧肾切除联合DOCA注射)与非手术组(单纯DOCA注射)模型的稳定性差异。方法将健康Wistar大鼠按体质量随机分为手术组与非手术组,手术组大鼠切除左肾后皮下注射DOCA(50 mg/kg)每周5次,共5周;非手术组无手术处理,同法皮下注射DOCA,每周5次,共7周。两组大鼠均自由饮水1%盐水,期间持续监测尾静脉血压至第9周。观察各组大鼠血压变化,并比较各组模型的成模率与稳定性。结果手术组大鼠给药后,血压逐步升高,停药后血压持续维持高位且波动轻微,形成持续性高血压;非手术组大鼠在同期给药及停药后,血压呈现与手术组一致的变化特征,即给药后逐步升高、停药后持续维持高血压状态。结论皮下注射DOCA可在不实施肾切除手术的条件下有效诱导大鼠高血压模型,与传统高血压模型相比,非手术造模方法更简便、安全、可重复,具备较高的应用推广价值。
Objective To evaluate the feasibility of establishing a hypertensive rat model induced by deoxycorticosterone acetate(DOCA),and to compare the stability of the model between surgery(unilateral nephrectomy combined with DOCA injection)and non-surgery group(DOCA injection alone).Methods Healthy Wistar rats were divided randomly into surgery group and non-surgery group according to body weight.Rats in surgery group underwent left nephrectomy followed by subcutaneous injection of DOCA(50 mg/kg),five times a week for 5 weeks.Rats in the non-surgery group received DOCA injections following the same protocol for 7 weeks,without nephrectomy.Both groups were given free access to 1%saline solution throughout the experiment.Tail artery blood pressure was monitored weekly until week 9.Blood pressure changes and model stability were compared between the groups.Results Following drug administration,the blood pressure of rats in the surgery group gradually increased.After drug withdrawal,the blood pressure remained persistently elevated with slight fluctuations,resulting in sustained hypertension.During the same period of drug administration and withdrawal,the blood pressure of rats in the nonsurgery group exhibited a consistent change pattern with that in the surgery group,i.e.,a gradual increase after drug administration and sustained maintenance of hypertension after drug withdrawal.Conclusions Subcutaneous DOCA administration can effectively induce hypertension in rats without the need for nephrectomy.Compared with the conventional surgical model,the non-surgical method is simpler,safer,and reproducible,and has broad applicability for hypertension research.
作者
王巍巍
刘潇然
孙振国
吴怡
王晓丽
李凤新
WANG Weiwei;LIU Xiaoran;SUN Zhenguo;WU Yi;WANG Xiaoli;LI Fengxin(Jilin Institute of Pharmaceutical Research,Changchun 130061,China)
出处
《中国比较医学杂志》
北大核心
2026年第2期21-29,共9页
Chinese Journal of Comparative Medicine
基金
吉林省科技发展计划项目(20240404033ZP)。
关键词
高血压
DOCA
动物模型
hypertension
deoxycorticosterone acetate
animal model
