摘要
目的:观察朱琏抑制Ⅱ型针法对缺血性中风(IS)大鼠神经功能和血管生成的影响,并探讨其潜在机制。方法:Wistar大鼠分为假手术组、模型组、针刺组、针刺+血管生成素(Ang)-2组,每组12只。采用线栓法制备IS模型。针刺组选取“关元”“归来”等穴行朱琏抑制Ⅱ型针法针刺,30 min/次,针刺+Ang-2组大鼠尾静脉注射大鼠Ang-2重组蛋白(10 mg/kg)后再进行朱琏抑制Ⅱ型针法干预,两组治疗均1次/d,连续7 d。对各组大鼠进行神经功能缺损评分;TTC染色测量大鼠脑梗死体积百分比;HE染色观察大鼠大脑缺血半暗带皮层病理形态变化;免疫组织化学法检测皮层微血管密度;Western blot法检测大鼠缺血半暗带大脑皮层血管内皮生长因子(VEGF)、血管内皮生长因子受体2(VEGFR-2)、Ang-1、Ang-2、酪氨酸激酶2(Tie-2)、磷酸化(p)Tie-2(p-Tie-2)蛋白表达。结果:与假手术组比较,模型组大鼠神经功能缺损评分、脑梗死体积百分比、缺血半暗带脑皮层微血管密度及VEGF、VEGFR-2、Ang-1蛋白表达水平及p-Tie-2/Tie-2比值升高(P<0.05),Ang-2蛋白表达水平降低(P<0.05)。与模型组和针刺+Ang-2组比较,针刺组大鼠神经功能缺损评分、脑梗死体积百分比、Ang-2蛋白表达水平降低(P<0.05),缺血半暗带皮层微血管密度及VEGF、VEGFR-2、Ang-1蛋白表达水平及p-Tie-2/Tie-2比值升高(P<0.05)。模型组大鼠缺血半暗带皮层神经元数量减少,核固缩或消失,胞体空泡化变性,并伴有大量炎性细胞浸润;与模型组比较,针刺组大鼠皮层神经元损伤减轻;与针刺组比较,针刺+Ang-2组大鼠皮层神经元损伤加重。结论:朱琏抑制Ⅱ型针法可改善IS大鼠的神经功能损伤,其机制可能与激活Ang-1/Tie-2通路促进血管新生有关。
Objective To observe the effects of Zhu Lian’s typeⅡinhibition needling on neurological function and angiogenesis in rats with ischemic stroke(IS),so as to explore its potential mechanisms.Methods Wistar rats were divided into sham operation group,model group,acupuncture group,and acupuncture+angiogenin(Ang)-2 group,with 12 rats in each group.A suture method was used to establish the IS model.The acupuncture group received Zhu Lian’s typeⅡinhibition needling at the“Guanyuan”(CV4),“Guilai”(ST29),“Zusanli”(ST36),“Sanyinjiao”(SP6),and“Fenglong”(ST40)acupoints for 30 min per session.Rats in the acupuncture+Ang-2 group were injected via the tail vein with Ang-2 recombinant protein(10 mg/kg)before Zhu Lian’s typeⅡinhibition needling intervention.All treatments were administered once daily for 7 consecutive days.Neurological deficit scores were assessed in rats of each group.TTC staining was employed to determine the percentage of cerebral infarction volume.HE staining was used to observe the pathological changes in the ischemic penumbra cortex.Immunohistochemistry was used to evaluate microvascular density in the ischemic penumbra cortex.Western blot was conducted to detect the expression levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR2),Ang-1,Ang-2,tyrosine kinase 2(Tie-2),and phosphorylated Tie-2(p-Tie-2)proteins in the ischemic penumbra cortex.Results Compared with the sham operation group,the model group showed elevated neurological deficit scores,cerebral infarction volume percentage,microvascular density in the ischemic penumbra cortex,and protein levels of VEGF,VEGFR-2,Ang-1,and p-Tie-2/Tie-2 ratio.Conversely,Ang-2 protein level was decreased(P<0.05).Compared with the model group and the acupuncture+Ang-2 group,rats in the acupuncture group had lower neurological deficit scores,cerebral infarction volume percentage,and Ang-2 protein level.While microvascular density in the ischemic penumbra cortex and the protein levels of VEGF,VEGFR-2,Ang-1,and the p-Tie-2/Tie-2 ratio were further increased(P<0.05).In the model group,the number of neurons in the ischemic penumbra cortex of rats was decreased,the nuclei were shrank or disappeared,the cell bodies were vacuolated and degenerated,accompanied by a large number of inflammatory cell infiltrations.Compared with the model group,the injury of cortical neurons in the acupuncture group was alleviated.Compared with the acupuncture group,the injury of cortical neurons in the acupuncture+Ang-2 group was aggravated.Conclusion Zhu Lian’s typeⅡinhibition needling can improve neurological function in IS rats,possibly by activating the Ang-1/Tie-2 pathway to promote angiogenesis.
作者
翟阳
黎芳
莫智珍
梅小平
韦玉鲁
池腾飞
邹敏
庞延
王瀚
ZHAI Yang;LI Fang;MO Zhi-zhen;MEI Xiao-ping;WEI Yu-lu;CHI Teng-fei;ZOU Min;PANG Yan;WANG Han(Department of Encephalopathy,Nanning Seventh People’s Hospital&Nanning Institute of Acupuncture and Moxibustion,Nanning 530012,China;Department of Area 1 of Acupuncture and Rehabilitation,Nanning Seventh People’s Hospital&Nanning Institute of Acupuncture and Moxibustion,Nanning 530012;Department of Encephalopathy,Guangxi International Zhuang Medical Hospital&Guangxi International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530200;Department of Pediatrics,Guangxi International Zhuang Medical Hospital&Guangxi International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530200;Department of Emergency,The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023)
出处
《针刺研究》
北大核心
2026年第1期31-38,共8页
Acupuncture Research
基金
广西壮族自治区中医药管理局自筹课题项目(No.GXZYA20230437)
中国博士后科学基金资助项目(No.2024MD753922)
广西自然科学基金项目(No.2024GXNSFAA010252)
广西青年岐黄学者培养项目(No.GXQH202419)。
