摘要
目的建立自动固相微萃取气相色谱-串联质谱法,测定生活饮用水中痕量2-甲基异莰醇(2-methylisoborneol,2-MIB)和土臭素(geosmin,GSM)。方法优化气相色谱条件和质谱参数提升仪器检测灵敏度,通过对比不同盐析条件、萃取温度、萃取时间获得最佳前处理及固相微萃取条件,并对加标回收率、精密度、方法检出限及定量限等方法性能进行评估。结果本研究建立的方法可准确测定5 mL生活饮用水中痕量2-MIB和GSM。在2.0~100 ng/L浓度范围内,2-MIB和GSM的线性关系良好,相关系数(r)均大于0.999。2-MIB和GSM的检出限分别为0.56和0.71 ng/L,定量限分别为1.84和2.36 ng/L,加标回收率范围分别为89.7%~115%和83.3%~110.3%,日内相对标准偏差范围分别为2.4%~3.1%和2.3%~4.7%,日间相对标准偏差范围分别为4.2%~9.4%和3.2%~7.5%。应用本方法对5份生活饮用水进行分析,两种目标物均有检出,检出范围分别为1.6~4.6和2.2~2.5 ng/L,均低于《生活饮用水卫生标准》(GB/T 5749-2022)中规定的限值(10 ng/L)。结论本方法操作简单,能够实现对生活饮用水中2-MIB和GSM的准确、高效、高灵敏度检测。
Objective To establish a method using automated solid-phase microextraction-gas chromatography-tandem mass spectrometry for the determination of trace levels of 2-methylisoborneol(2-MIB)and geosmin(GSM)in drinking water.Methods The sensitivity of the instrument was enhanced by optimizing gas chromatography conditions and mass spectrometry parameters.Various salting-out conditions,extraction temperatures,and extraction times were compared to identify the optimal pretreatment and solid-phase microextraction conditions.The performance was evaluated based on spiked recovery,precision,limit of detection,and limit of quantification.Results The established method enabled the accurate determination of trace 2-MIB and GSM in 5 mL drinking water samples.The method demonstrated excellent linearity for both 2-MIB and GSM within the concentration range of 2.0 to 100 ng/L,with correlation coefficients(r)exceeding 0.999.The limits of detection for 2-MIB and GSM were 0.56 and 0.71 ng/L,respectively,and their limits of quantitation were 1.84 and 2.36 ng/L,respectively.The spiked recoveries ranged from 89.7%to 115%for 2-MIB and from 83.3%to 110.3%for GSM.The intra-day relative standard deviations for 2-MIB and GSM ranged from 2.4%to 3.1%and from 2.3%to 4.7%,respectively.The inter-day relative standard deviations ranged from 4.2%to 9.4%and from 3.2%to 7.5%,respectively.This method was applied to analyze five drinking water samples and both target compounds were detected.Their concentrations ranged from 1.6 to 4.6 ng/L(2-MIB)and from 2.2 to 2.5 ng/L(GSM).These concentrations were below the limit of 10 ng/L as specified in the Standards for Drinking Water Quality(GB/T 5749-2022).Conclusion This method is simple,accurate,efficient,and highly sensitive for the determination of 2-MIB and GSM in drinking water.
作者
鲍珊
白雪
蒋宇晨
邱天
陆一夫
BAO Shan;BAI Xue;JIANG Yu-chen;QIU Tian;LU Yi-fu(China CDC Key Laboratory of Environment and Population Health/National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;Center for Global Health,School of Public Health,Nanjing Medical University)
出处
《环境卫生学杂志》
2026年第1期74-79,共6页
JOURNAL OF ENVIRONMENTAL HYGIENE
基金
科技基础资源调查专项(2019FY101101)。
关键词
自动固相微萃取
气相色谱-串联质谱
2-甲基异莰醇
土臭素
生活饮用水
automated solid-phase microextraction
gas chromatography-tandem mass spectrometry
2-methylisoborneol
geosmin
drinking water
