摘要
【目的】RGLG蛋白属于RING(Really Interesting New Gene)型E3泛素连接酶,通过泛素化降解其他蛋白参与植物生长发育和非生物胁迫响应。鉴定甜瓜CmRGLG基因家族(CmRGLGs)成员,并分析相关基因的表达模式,为进一步探究其潜在功能奠定理论基础。【方法】采用生物信息学方法对CmRGLGs染色体定位、基因结构及其编码蛋白的理化特性、系统进化、共线性关系、蛋白互作等方面进行分析,通过RT-qPCR对各成员在不同器官、不同激素浓度梯度和非生物胁迫处理后甜瓜幼叶中的表达水平进行解析。【结果】在甜瓜全基因组中共鉴定出6个CmRGLGs成员,根据各基因在染色体上的位置及排列顺序依次命名为CmRGLG1-CmRGLG6;所编码的蛋白均为亲水性蛋白,氨基酸数为364-596 aa;在进行系统进化分析时,CmRGLGs基因分属于不同的3个分支。种内与种间共线性分析表明,CmRGLGs中不存在基因重复事件,CmRGLG1、CmRGLG2、CmRGLG4、CmRGLG5在拟南芥、黄瓜、番茄中均存在共线基因;启动子区中存在与植物激素和非生物胁迫响应有关的顺式作用元件;蛋白互作网络预测分析提示,CmRGLGs相互作用的蛋白主要富集在泛素-蛋白转移酶活性、蛋白质代谢、生物合成和有机环状化合物结合等途径;表达特性分析结果显示,CmRGLG5和CmRGLG6在茎中的表达量最低,CmRGLG1、CmRGLG2、CmRGLG3、CmRGLG4在花中的表达量较高,且各基因成员在不同植物激素和非生物胁迫中具有不同程度的表达。【结论】甜瓜CmRGLGs在不同植物激素处理条件下,多数成员的表达水平有下调趋势;在非生物胁迫处理条件下,多数成员的表达水平呈先上调再下调的趋势。
【Objective】RGLG proteins belong to the RING(Really Interesting New Gene,RING)type E3 ubiquitin ligases,which participate in plant growth,development,and abiotic stress responses by ubiquitinating and degrading other proteins.By identifying the members of the CmRGLGs gene family(CmRGLGs)in melon and analyzing their expression patterns,this study lays a theoretical foundation for further investigation into their potential functions.【Method】Bioinformatics methods were employed to analyze the chromosomal localization,gene structure,physicochemical properties of encoded proteins,phylogenetic evolution,syntenic relationships,and protein-protein interactions of CmRGLGs.The expression levels of these genes in different organs,under varying phytohormone concentrations,and after abiotic stress treatments were analyzed using RT-qPCR in young melon leaves.【Result】A total of six CmRGLGs members(CmRGLG1-CmRGLG6)were identified in the melon genome,named based on their chromosomal positions.The encoded proteins were hydrophilic,with amino acid lengths ranging from 364 to 596 aa.Phylogenetic analysis classified CmRGLGs into three distinct branches.Intraspecific and interspecific collinearity analysis revealed no gene duplication events among CmRGLGs.CmRGLG1,CmRGLG2,CmRGLG4,and CmRGLG5 showed collinear genes in Arabidopsis thaliana,Cucumis sativus,and Solanum lycopersicum.The promoter regions contained cis-acting elements associated with phytohormones and abiotic stress responses.Protein-protein interaction network prediction revealed that CmRGLGs-interacting proteins were primarily enriched in ubiquitin-protein transferase activity,protein metabolism,biosynthesis,and organic cyclic compound binding pathways.Expression analysis showed that CmRGLG5 and CmRGLG6 were expressed in the stems at the lowest level,while CmRGLG1,CmRGLG2,CmRGLG3,and CmRGLG4 were more abundant in the flowers.Additionally,each gene presented varying expression under different phytohormone treatments and abiotic stress conditions.【Conclusion】Under different phytohormone treatments,most CmRGLG members in melon showed downregulated expression trends.Under abiotic stress treatments,the majority of members demonstrated an initial upregulation followed by downregulation in their expressions.
作者
王亚萍
金兰
郝金凤
长明
王艳丹
高峰
WANG Ya-ping;JIN Lan;HAO Jin-feng;CHANG Ming;WANG Yan-dan;GAO Feng(College of Life Sciences and Technology,Inner Mongolia Normal University,Key Laboratory of Biodiversity Conservation and Sustainable Uti-lization for Mongolian Plateau in Higher Education Institutions of Inner Mongolia Autonomous Region,Hohhot 010022;School of Life Sci-ences,Inner Mongolia University,Key Laboratory of Biology of Forage and Characteristic Crops,Ministry of Education,Hohhot 010021)
出处
《生物技术通报》
北大核心
2025年第12期156-167,共12页
Biotechnology Bulletin
基金
国家自然科学基金项目(31660577,32360765)
内蒙古师范大学引进高层次人才科研启动金专项资金资助(2025YJRC083),内蒙古师范大学学生科技创新项目(2025XSKC16)。
关键词
甜瓜
泛素连接酶
RGLG基因家族
生物信息学
蛋白互作
植物激素
非生物胁迫
表达分析
Cucumis melo L.
ubiquitin ligase
RGLG gene family
bioinformatics
protein-protein interaction
phytohormones
abiotic stress
expression analysis
