摘要
原叶绿素酸酯氧化还原酶(protochlorophyllide oxidoreductase, POR)是叶绿素合成的必要酶,在叶绿素代谢中发挥重要作用。本研究基于公开的茶树基因组数据鉴定得到4个CsPORs基因,并对其进行生物信息学分析和表达分析。结果表明,4个CsPORs基因CDS全长为1 200~1 368 bp,编码蛋白均以碱性氨基酸为主,均为稳定的亲水性蛋白。CsPOR1、CsPOR2、CsPOR3蛋白仅含有cl21454结构域,而CsPOR4同时含有cl21454与cl47985结构域,CsPOR1、CsPOR2、CsPOR3具有较近的亲缘关系。CsPORs蛋白定位在叶绿体,不存在跨膜结构,不含信号肽;蛋白二级结构主要是α-螺旋和无规卷曲,三级结构相似性高。CsPORs基因的启动子含有多个光调控、植物激素和逆境胁迫响应元件。此外,CsPORs在茶树不同组织以及不同叶色茶树中的表达结果显示,CsPORs主要在茶树叶片中表达,其表达水平会影响茶树叶片呈色。本研究结果可为茶树CsPOR基因家族生物学功能的深入研究提供理论依据。
Protochlorophyllide oxidoreductase(POR)is an essential enzyme for chlorophyll synthesis,which plays the key role in chlorophyll metabolism.In this study,four CsPOR genes were identified based on the whole genome data of tea plant(Camellia sinensis),and the bioinformatics analysis and gene expression were carried out.The results showed that the CDS length of the four CsPOR genes was in the range of 1200 and 1368 bp,and the encoded proteins dominated by basic amino acids were stable and hydrophilic proteins.The sequence of CsPOR1,CsPOR2 and CsPOR3 proteins contained only cl21454 domain,while the se-quences of CsPOR4 contained cl21454 and cl47985 domains.CsPOR1,CsPOR2 and CsPOR3 had close ge-netic relationships.CsPOR proteins located in chloroplast,and had no transmembrane structure and signal peptide;their secondary structure was mainly composed ofα-helix and random coil,and their tertiary structure was similar.The promoter of CsPOR genes contained many elements of light regulation,plant hormones and stress response.Furthermore,the expression of CsPORs in different tissues and tea plants with different leaf colors showed that the CsPORs mainly expressed in tea leaves,and the expression level affected the coloration of tea leaves.This study provided a theoretical basis for the further study of biological functions of CsPOR gene family.
作者
张向娜
童杰文
陈凌芝
张宁宁
尹鹏
刘威
郭桂义
Zhang Xiangna;Tong Jiewen;Chen Lingzhi;Zhang Ningning;Yin Peng;Liu Wei;Guo Guiyi(College of Tea Science,Xinyang Agriculture and Forestry University/Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan,Xinyang 464000,China)
出处
《山东农业科学》
北大核心
2025年第11期10-18,共9页
Shandong Agricultural Sciences
基金
河南省高等学校重点科研项目(24A210024)
河南省自然科学基金项目(252300420727)
河南省科技攻关项目(252102110308)
信阳农林学院青年教师科研基金项目(QN2022008)。
关键词
茶树
原叶绿素酸酯氧化还原酶
基因家族
生物信息学分析
表达分析
Tea plant(Camellia sinensis)
Protochlorophyllide oxidoreductase(POR)
Gene family
Bioinformatics analysis
Expression analysis
