摘要
【目的】探究内生真菌哈茨木霉(Trichoderma harzianum Rifai)对珍稀药用植物米槁[Camphora migao(H.W.Li)Y.Yang,Bing Liu&Zhi Yang]幼苗抵御干旱胁迫的作用机制。【方法】对米槁幼苗进行根际注菌,接种哈茨木霉后采用盆栽称重法模拟不同干旱胁迫进行处理,探究植株生长及生理生化指标的响应情况。【结果】在不同干旱条件下,与未接种菌的对照组相比,接种哈茨木霉显著提高了米槁幼苗的生物量、株高和根系生长等各项生长指标;显著提高了幼苗叶片抗氧化酶活性和渗透调节物质含量,降低了叶片丙二醛含量,提升了光合色素含量,有效缓解了水分亏缺对幼苗生长发育的胁迫效应。【结论】哈茨木霉通过提高米槁幼苗调节渗透平衡、保持抗氧化系统稳定等应对干旱胁迫的生理能力,进而协助米槁幼苗有效应对干旱逆境。
[Objective]To investigate the mechanism by which the endophytic fungus Trichoderma harzianum Rifai help the seedlings of the rare medicinal plant Camphora migao(H.W.Li)Y.Yang,Bing Liu&Zhi Yang to defend against drought stress.[Methods]We simulated different drought stress gradients using the potted weighing method after inoculation of C.migao seedlings with T.harzianum via rhizosphere injection and investigated the plant growth,physiological,and biochemical indexes.[Results]Under different drought conditions,inoculation with T.harzianum significantly increased the growth indexes such as biomass,plant height,and root growth of C.migao seedlings,compared with the uninoculated control group.Furthermore,T.harzianum significantly increased the activities of antioxidant enzymes and the content of osmotically regulation substances,reduced the malondialdehyde content,and elevated the content of photosynthetic pigments in the seedling leaves,which effectively mitigated the drought stress effects on the growth and development of C.migao seedlings.[Conclusion]T.harzianum improved the physiological responses of C.migao seedlings to drought stress by regulating osmotic balance and maintaining the stability of antioxidant system.
作者
王倩
陈敬忠
廖小锋
谢元贵
张兰
张曼华
彭天慧
WANG Qian;CHEN Jingzhong;LIAO Xiaofeng;XIE Yuangui;ZHANG Lan;ZHANG Manhua;PENG Tianhui(College of Forestry,Guizhou University,Guiyang,Guizhou,China;College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou,China;Guizhou Botanical Garden,Guiyang,Guizhou,China;Guizhou Institute of Mountain Resources,Guiyang,Guizhou,China;Guizhou Academy of Forestry,Guiyang,Guizhou,China)
出处
《微生物学报》
北大核心
2025年第12期5540-5558,共19页
Acta Microbiologica Sinica
基金
贵州省科技支撑计划(黔科合支撑[2023]一般049)
贵州省教育厅自然科学研究项目(青年科技人才成长项目)(黔教技[2024]121号)
贵州省基础研究计划(黔科合基础-ZK[2025]面上176)
贵州省林业局科技计划(黔林科合[2025]03号)。
关键词
哈茨木霉
米槁
干旱胁迫
生长发育
生理代谢
Trichoderma harzianum
Camphora migao
drought stress
growth and development
physiological metabolism
