摘要
最早从拟南芥(Arabidopsisthaliana)中克隆到的NPR1(nonexpressorofpathogenesis_relatedgenes1)基因是调控植物病害抗性的一个关键基因。它不仅对植物系统获得抗性(systemicacquiredresistance,SAR)和诱导系统抗性(inducedsystemicresistance,ISR)起核心调控作用,而且是植物基础抗性(basicresistance)以及由抗病基因(resistancegene,R)决定的抗性的重要调控因子。氧化突发(oxidativeburst)造成的强还原势导致NPR1蛋白还原成单体,以及NPR1单体在细胞核内的积累是诱导水杨酸(salicylicacid,SA)介导的PR(pathogenesis_related)基因表达和SAR产生的充分必要条件。NPR1通过与TGA转录因子的相互作用调控PR基因表达。NPR1作为多种信号途径的交叉点,与某些WRKY转录因子和NPR4一起,在调节和平衡SA和茉莉酸信号传导途径中起关键作用。NPR1的这种调控作用在细胞质内进行,通过遗传工程将其用于植物保护有很好的应用前景。
The NPR1 (nonexpressor of pathogenesis-related genes 1) gene, firstly cloned in Arabidopsis thaliana, is a key gene involved in regulation of plant disease resistance. It plays a pivotal role not only in systemic acquired resistance (SAR) and induced systemic resistance (ISR), but also in basic resistance and resistance (R) gene-dependent resistance. NPR1 monomerization induced by enhanced reducing condition after oxidative burst, and the accumulation of NPR1 monomers in the nuclei, are required and enough for expression of PR (pathogenesis-related) genes and SAR. NPR1 regulates PR gene expression through interaction with TGA transcription factors (TF). As a cross-talk point of a variety of defense signaling pathways, probably through direct or indirect interacting with some WRKY TFs and a NPR1-like protein NPR4, NPR1 is essential in balancing salicylic acid- and jasmonic acid- dependent signal transduction pathways, which is achieved through an unknown mechanism in the cytosol. The possible application of NPR1 in plant protection is also discussed in this review.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第4期511-515,共5页
Chinese Journal of Biotechnology
基金
国家自然科学基金资助项目(No.30040792
30170597)~~
关键词
NPR1
抗病性
防卫反应
信号传导
水杨酸
乙烯
茉莉酸
NPR1, disease resistance, defense response, signal transduction, salicylic acid, ethylene, jasmonic acid
