摘要
为深入了解低温胁迫条件下刀鲚肌肉组织的蛋白表达情况,本研究以体质量为(1.99±1.50)g的刀鲚幼鱼为研究对象,采用astral_DIA技术对急性低温胁迫条件下刀鲚肌肉组织进行蛋白质组学分析。通过数据库检索,共鉴定到3100个肌肉组织蛋白,其中差异表达蛋白134个,包括表达上调蛋白54个,表达下调蛋白80个。生物信息学分析发现,这些差异表达蛋白主要参与了丙氨酸、天冬氨酸和谷氨酸代谢、TGF-β信号通路、昼夜节律、p53信号通路等,主要涉及细胞过程、代谢、环境信息处理等生物学过程。组织切片分析表明,急性低温胁迫会对刀鲚肌肉组织结构产生损伤。本研究结果可为进一步深入了解刀鲚在低温胁迫下的适应性调节机制及耐低温鱼类优良品种的培育提供理论依据。
In order to an in-depth understanding of the protein expression profile in the muscle tissue of the Coilia nasus under low-temperature stress,this study used C.nasus with a body weight of(1.99±1.50)g as the research subjects,and carried out proteomics analysis of the muscle tissues of juvenile C.nasus under the condition of acute low-temperature stress was carried out by using the astral_DIA technology.Through database retrieval,a total of 3,100 muscle tissue proteins were identified,including 134 differentially expressed proteins,including 54 upregulated proteins and 80 downregulated proteins.Through bioinformatics analysis,it was found that these differentially expressed proteins are mainly involved in pathways such as alanine,aspartate,and glutamate metabolism,TGF-βsignaling pathway,circadian rhythm,and p53 signaling pathway.These pathways were mainly related to biological processes,such as cellular processes,metabolism,and environmental information processing.Tissue section analysis demonstrated that acute low temperature stress could damage the muscle tissue structure of C.nasus.The results of this study provide a theoretical basis for further in-depth understanding of the adaptive regulatory mechanism of C.nasus under low-temperature stress and the cultivation of excellent varieties of cold-tolerant fish.
作者
朱春月
刘忠航
张伯序
胡宗云
张健
杨培民
ZHU Chunyue;LIU Zhonghang;ZHANG Boxu;HU Zongyun;ZHANG Jian;YANG Peimin(Liaoning Institute of Freshwater Fisheries,Liaoning Key Laboratory of Aquatic Animal Disease Control,Liaoyang 111000,China)
出处
《水产学杂志》
2025年第6期20-28,共9页
Chinese Journal of Fisheries
基金
辽宁省农科院院长基金项目(2024QN1818)
辽宁省农科院项目(2020XKHBZ0706、2021HQ1918)
辽宁省科技厅项目(2021JH2/10200031)
辽宁省农业科学院“特色淡水鱼”学科建设专项(2024XKJS5250)。
关键词
刀鲚
低温
肌肉
蛋白组学
Coilia nasus
low temperature
muscle
proteomics
