摘要
目的建立食品中依托咪酯类新精神活性物质的非靶向筛查与测定方法。方法采用超高效液相色谱-串联高分辨质谱法对公安执法缴获的食品样品进行依托咪酯类似物筛查,并通过傅里叶变换核磁共振氢谱对该物质进行了结构表征,确证该化合物为新精神活性药物异丙帕酯。该物质与依托咪酯互为同系物。结果利用所建方法,在食品样品中成功发现新型精神活性物质异丙帕酯并建立依托咪酯、美托咪酯及异丙帕酯的定量方法,3种目标物在2.00~50.00µg/L范围内线性关系良好,检出限均为0.05 mg/kg,定量限均为0.10 mg/kg,在压片糖果基质中3个加标水平下的平均回收率为89.6%~104.0%,相对标准偏差(n=6)为1.7%~4.2%。结论本方法前处理简便、灵敏度高、准确性好,适用于食品中依托咪酯类化合物的非靶向筛查与定量检测,可为新型精神活性物质的监管提供技术支撑。
Objective To establish a non-targeted screening and quantitative method for etomidate-type novel psychoactive substances in food.Methods Food samples seized in law-enforcement operations were screened for etomidate analogues by ultra performance liquid chromatography-tandem high-resolution mass spectrometry.The structure of the newly detected compound was elucidated and confirmed by 1H Fourier-transform nuclear magnetic resonance as isopropoxate,a novel psychoactive drug that was homologous to etomidate.Results Using the developed method,isopropoxate was identified in seized food products.Three kinds of quantitative methods for etomidate-type new psychoactive substances were established.Good linearity was obtained in the range of 2.00-50.00μg/L.The limits of detection and limits of quantitation were 0.05 mg/kg and 0.10 mg/kg,respectively.In compressed-candy matrix,mean recoveries at 3 spiking levels ranged from 89.6%to 104.0%,with relative standard deviations(n=6)of 1.7%-4.2%.Conclusion The method is characterized by simple sample preparation,high sensitivity and excellent accuracy,and is suitable for the non-targeted screening and determination of etomidate-type compounds in food samples.
作者
何敏恒
李秀英
林晓佳
周焯君
陈扬
HE Min-Heng;LI Xiu-Ying;LIN Xiao-Jia;ZHOU Zhuo-Jun;CHEN Yang(Guangzhou Inspection Testing and Certification Group Co.,Ltd.,Guangzhou 511447,China;National Quality Inspection and Testing Center for Processed Foods(Guangdong),Guangzhou 511447,China)
出处
《食品安全质量检测学报》
2025年第24期140-147,共8页
Journal of Food Safety and Quality
基金
广东省市场监督管理局科技项目(2023CS03)。
关键词
非靶向筛查
依托咪酯
超高效液相色谱-串联高分辨质谱法
非法添加物
non-targeted screening
etomidate
ultra performance liquid chromatography-tandem high-resolution mass spectrometry
illegal additives
