摘要
目的探讨错配修复蛋白和黏蛋白2、黏蛋白6表达与胃癌的预后及免疫浸润的相关性。方法回顾性选取2019年2月至2020年2月入宁夏医科大学总医院接受手术切除治疗的胃癌患者110例,收集患者胃癌样本以及癌旁5 cm外的癌旁组织样本。比较胃癌组织与癌旁组织的错配修复蛋白和黏蛋白2、黏蛋白6蛋白表达的差异,分析其蛋白表达与不良预后、5年生存率及免疫浸润的关系。结果胃癌组织错配修复蛋白缺失发生率为32.72%,高于癌旁组织(8.18%),黏蛋白2蛋白阳性表达率为39.09%,高于癌旁组织(17.27%),黏蛋白6蛋白阳性表达率为51.81%,低于癌旁组织(78.18%),差异均有统计学意义(P<0.05)。不同分化程度、TNM分期、有无局部浸润、有无淋巴结转移的胃癌患者错配修复蛋白缺失发生率及黏蛋白2和黏蛋白6蛋白阳性表达率比较,差异均有统计学意义(P<0.05)。胃癌组织错配修复蛋白缺失患者5年生存率为19.44%,低于错配修复蛋白完整患者5年生存率(54.05%),差异有统计学意义(P<0.05);胃癌组织黏蛋白2阳性患者5年生存率为25.58%,低于黏蛋白2阴性患者5年生存率(53.73%),差异有统计学意义(P<0.05);胃癌组织黏蛋白6阳性患者5年生存率为57.89%,高于黏蛋白6阴性患者5年生存率(26.42%),差异有统计学意义(P<0.05)。分化程度、TNM分期、局部浸润、淋巴结转移、错配修复蛋白、黏蛋白6、黏蛋白2均是胃癌患者预后的独立影响因素(P<0.05)。Pearson相关性分析结果显示,错配修复蛋白、黏蛋白2基因表达与CD4^(+)、CD8^(+)、CD80^(+)和CD86^(+)均呈正相关(P<0.05),与CD68^(+)、CD115^(+)、CD163^(+)、Foxp3^(+)均呈负相关(P<0.05),黏蛋白6基因表达与CD4^(+)、CD8^(+)、CD80^(+)和CD86^(+)均呈负相关(P<0.05),与CD68^(+)、CD115^(+)、CD163^(+)、Foxp3^(+)均呈正相关(P<0.05)。结论胃癌中错配修复蛋白和黏蛋白2、黏蛋白6蛋白表达与其临床病理特征、不良预后及免疫浸润水平关系密切,可能参与免疫细胞调节。
Objective To explore the correlation between mismatch repair protein and the expression of mucin 2 and mucin 6 proteins with poor prognosis and immune infiltration in gastric cancer.Methods A total of 110 patients with gastric cancer patients who underwent surgical resection treatment at General Hospital of Ningxia Medical University from February 2019 to February 2020 were retrospectively selected,the gastric cancer samples and adjacent tissue samples 5 cm away from the cancer of patients were collected.Immunohistochemical staining was performed to detect the expression of mismatch repair protein,mucin 2,and mucin 6 proteins,and the relationship between their protein expression and poor prognosis,5-year survival rate,and immune infiltration were analyzed.Results The incidence of mismatch repair protein deletion in gastric cancer tissues was 32.72%,which was higher than that in adjacent tissues(8.18%),the positive expression rate of mucin 2 protein in gastric cancer tissues was 39.09%,which was higher than that in adjacent tissues(17.27%),the positive expression rate of mucin 6 protein in gastric cancer tissues was 51.81%,which was lower than that in adjacent tissues(78.18%),and the differences were statistically significant(P<0.05).The comparison of mismatch repair protein deficiency expression rates and positive expression rates of mucin 2 and mucin 6 proteins in gastric cancer patients with different degrees of differentiation,TNM staging,presence of local invasion,and lymph node metastasis shows statistically significant differences,the differences were statistical significance(P<0.05).The 5-year survival rate of gastric cancer patients with mismatch repair protein deficiency was 19.44%,which was lower than the 5-year survival rate of mismatch repair protein intact patients(54.05%),the difference was statistically significant(P<0.05).The 5-year survival rate of mucin 2 positive gastric cancer patients was 25.58%,which was lower than the 5-year survival rate of mucin 2 negative patients(53.73%),the difference was statistically significant(P<0.05);the 5-year survival rate of mucin 6 positive gastric cancer patients was 57.89%,which was higher than the 5-year survival rate of mucin negative patients(26.42%),the difference was statistically significant(P<0.05).The degree of differentiation,TNM staging,local invasion,lymph node metastasis,mismatch repair protein,mucin 6,and mucin 2 were independent factors affecting the prognosis of gastric cancer patients(P<0.05).Pearson correlation analysis showed that the expression of mismatch repair protein and mucin 2 proteins is positively correlated with CD4^(+),CD8^(+),CD80^(+),and CD86^(+)(P<0.05),and negatively correlated with CD68^(+),CD115^(+),CD163^(+),and Foxp3^(+)(P<0.05).The expression of mucin 6 protein is negatively correlated with CD4^(+),CD8^(+),CD80^(+),and CD86^(+)(P<0.05),and positively correlated with CD68^(+),CD115^(+),CD163^(+),and Foxp3^(+)(P<0.05).Conclusion The expression of mismatch repair protein and mucin 2,mucin 6 proteins in gastric cancer is closely related to clinical pathological features,poor prognosis,and levels of immune infiltration,and may participate in the regulation of immune cells.
作者
严慧
胡萍
黄英
勉睿清
YAN Hui;HU Ping;HUANG Ying(Department of Medicine Oncology,General Hospital of Ningxia Medical University,Yinchuan Ningxia 750004,China)
出处
《临床和实验医学杂志》
2025年第21期2249-2254,共6页
Journal of Clinical and Experimental Medicine
基金
宁夏科技厅项目-自治区重点研发计划项目(编号:2021BEG03082)。
关键词
胃肿瘤
预后
错配修复蛋白
黏蛋白2
黏蛋白6
免疫浸润
生存率
Gastric neoplasms
Prognosis
Mismatch repair protein
Mucin 2
Mucin 6
Immune infiltration
Survival rate
