摘要
本试验旨在研究不同浓度植物乳杆菌ZHR8后生元对断奶仔猪生长性能、肠道炎症、盲肠及结肠微生物的影响。选取120头初始体重为(10.46±0.22)kg的“杜×长×大”三元杂交断奶仔猪,随机分为4组:CON组(基础饲粮)、0.05%ZHR8组(基础饲粮+0.05%植物乳杆菌ZHR8后生元)、0.10%ZHR8组(基础饲粮+0.10%植物乳杆菌ZHR8后生元)、0.20%ZHR8组(基础饲粮+0.20%植物乳杆菌ZHR8后生元),每组3个重复,每个重复10头猪(公母各占1/2)。试验期29 d。结果表明:1)与CON组相比,0.10%ZHR8组断奶仔猪平均日增重显著升高(P<0.05),0.20%ZHR8组断奶仔猪平均日增重极显著升高(P<0.01);0.20%ZHR8组断奶仔猪末重显著升高(P<0.05),料重比显著降低(P<0.05)。2)与CON组相比,0.20%ZHR8组断奶仔猪空肠绒毛高度极显著升高(P<0.01);0.10%ZHR8、0.20%ZHR8组断奶仔猪空肠绒隐比显著升高(P<0.05)。3)与CON组相比,0.10%ZHR8组断奶仔猪结肠黏膜促炎因子白细胞介素-8(IL-8)、白细胞介素-12(IL-12)mRNA相对表达量显著降低(P<0.05);0.20%ZHR8组促炎因子干扰素-γ(IFN-γ)、IL-8 mRNA相对表达量显著降低(P<0.05),抗炎因子白细胞介素-10(IL-10)mRNA相对表达量显著升高(P<0.05)。4)与CON组相比,0.10%ZHR8组断奶仔猪结肠内容物戊酸含量极显著升高(P<0.01);0.20%ZHR8组戊酸、异戊酸含量极显著升高(P<0.01)。5)16S rRNA结果显示,在属水平上,与CON组相比,0.10%ZHR8、0.20%ZHR8组断奶仔猪结肠普雷沃氏菌科_NK3B31群相对丰度显著降低(P<0.05),拟普雷沃氏菌属相对丰度显著升高(P<0.05);0.10%ZHR8组普雷沃氏菌科_UCG-003相对丰度显著升高(P<0.05)。综上所述,在基础饲粮中添加0.10%~0.20%植物乳杆菌ZHR8后生元可提高断奶仔猪的生长性能,减少肠道炎症,增加肠道内容物挥发性脂肪酸含量,改善肠道微生物结构,从而增强断奶仔猪的肠道健康。
The purpose of this study was to investigate the effects of different concentrations of Lactobacillus plantarum ZHR8 postbiotics on growth performance,intestinal inflammation,and colonic microorganism in weaned piglets.A total of 120“Duroc×Landrace×Yorkshire”weaned piglets(half male and half female)with an initial body weight of(10.46±0.22)kg were randomly allocated into 4 groups(3 replicates per group,10 piglets per replicate):CON group(basic diet),0.05%ZHR8 group(basic diet+0.05%ZHR8 postbiotic),0.10%ZHR8 group(basic diet+0.10%ZHR8 postbiotic),0.20%ZHR8 group(basic diet+0.20%ZHR8 postbiotic),and the trial period was 29 days.The results showed that:1)compared to the CON group,the average daily gain of weaned piglets in the 0.10%ZHR8 group was significantly increased(P<0.05),and that in the 0.20%ZHR8 group was extremely significantly increased(P<0.01);the final weight of weaned piglets in the 0.20%ZHR8 group was significantly increased(P<0.05),and the feed to gain ratio was significantly decreased(P<0.05).2)Compared to the CON group,the villus height of the jejunum in the 0.20%ZHR8 group of weaned piglets were increased extremely significantly(P<0.01);the villus/crypt ratio of the jejunum in the 0.10%ZHR8 and 0.2%ZHR8 groups of weaned piglets was significantly increased(P<0.05).3)Compared with the CON group,the relative mRNA expression levels of pro-inflammatory factors interleukin-8(IL-8)and interleukin-12(IL-12)in the colonic mucosa of weaned piglets in the 0.10%ZHR8 group were significantly decreased(P<0.05);in the 0.20%ZHR8 group,the relative mRNA expression levels of pro-inflammatory factors interferon-γ(IFN-γ)and IL-8 were significantly decreased(P<0.05),while the relative mRNA expression level of anti-inflammatory factor interleukin-10(IL-10)was significantly increased(P<0.05).4)Compared to the CON group,the content of valeric acid in the colonic contents of weaned piglets in the 0.10%ZHR8 group was extremely significantly increased(P<0.01);The contents of valeric acid and isovaleric acid in the 0.20%ZHR8 group was extremely significantly increased(P<0.01).5)The results of 16S rRNA showed that at the genus level,the relative abundance of colon Prevotellaceae_NK3B31_group in the 0.10%ZHR8 and 0.20%ZHR8 groups was significantly decreased(P<0.05),the relative abundance of Alloprevotella was significantly increased(P<0.05),and the relative abundance of Prevotellaceae_UCG-003 in the 0.10%ZHR8 group was significantly increased(P<0.05).Adding 0.10%to 0.20%Lactobacillus plantarum ZHR8 postbiotics to the basal diet can improve the growth performance of weaned piglets,reduce intestinal inflammation,increase the content of volatile fatty acids in intestinal contents,and improve intestinal microbial structure,thereby enhancing the intestinal health of weaned piglets.
作者
王媛媛
宦海琳
马清泉
许思研
刘能文
闫俊书
WANG Yuanyuan;HUAN Hailin;MA Qingquan;XU Siyan;LIU Nengwen;YAN Junshu(Key Laboratory of Crop and Livestock Integrated Farming,Ministry of Agriculture and Rural Affairs,Institute of Animal Husbandry,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China)
出处
《动物营养学报》
北大核心
2025年第11期7416-7428,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
江苏省农业科技自主创新资金“提升仔猪肠道免疫机能的微生态调控关键技术研发”
国家重点研发计划“畜禽胃肠道和生殖道健康与营养调控技术”(2022YFD1300400)。
关键词
植物乳杆菌ZHR8
后生元
断奶仔猪
肠道炎症
微生物菌群
Lactobacillus plantarum ZHR8
postbiotics
weaned piglets
intestinal inflammation
microorganism
