摘要
通过以纤维素酶对簕菜进行处理,并采用大孔吸附树脂加以纯化,制备出一种簕菜提取物。为了研究簕菜提取物中的活性成分含量,以及簕菜提取物的体外抗氧化活性,该研究采用高效液相色谱法对簕菜提取物中的绿原酸、隐绿原酸、芦丁、异绿原酸A、异绿原酸B、异绿原酸C等多酚类物质进行测定,并评价了簕菜提取物对DPPH自由基和ABTS^(+)自由基的清除能力。结果表明,簕菜提取物中6种活性成分的总含量为31.76%,其中绿原酸的含量最高;簕菜提取物对DPPH自由基和ABTS^(+)自由基清除能力的IC_(50)值分别为13.91μg/mL、26.22μg/mL,具有良好的抗氧化活性。该研究为簕菜的开发利用提供了理论依据。
By treating Acanthopanax trifoliatus with cellulase and purifying it using macroporous adsorption resin,an extract of Acanthopanax trifoliatus has been prepared.In order to study the content of active components in the extract of Acanthopanax trifoliatus and evaluate its in vitro antioxidant activity,in this study,high performance liquid chromatography(HPLC)was used to determine the content of chlorogenic acid(CA),4-dicaffeoylquinic acid(4DA),rutin(Rut),isochlorogenic acid A(IAA),isochlorogenic acid B(IAB),isochlorogenic acid C(IAC)in the extract of Acanthopanax trifoliatus.The ability of the extract of Acanthopanax trifoliatus to scavenge DPPH and ABTS^(+)free radicals was studied.The results showed that the total content of 6 active components in the extract of Acanthopanax trifoliatus was 31.76%,of which chlorogenic acid had the highest content;The IC_(50)values of the DPPH free radical and ABTS^(+)free radical scavenging abilities of the extract from Acanthopanax trifoliatus were 13.91μg/mL and 26.22μg/mL,respectively,exhibiting good antioxidant activity.This study provides theoretical basis for the development and utilization of the extract of Acanthopanax trifoliatus.
作者
李嘉俊
黄燕秋
苏汝彬
赵斌
彭荣珍
蔡佳梓
吴嘉燕
黄方喆
文艺璇
LI Jiajun;HUANG Yanqiu;SU Rubin;ZHAO Bin;PENG Rongzhen;CAI Jiazi;WU Jiayan;HUANG Fangzhe;WEN Yixuan(Guangdong Jiangmen Chinese Medicine College/Jiangmen Health Products Branch Center,National Engineering Technology Research Center for Modernization of Traditional Chinese Medicine,Jiangmen 529000,China;Jiangmen Engineering Technology Research Center of Health Products,Jiangmen 529000,China)
出处
《食品与发酵科技》
2025年第6期119-124,共6页
Food and Fermentation Science & Technology
基金
广东省普通高校青年创新人才项目(2020KQNCX231)
广东江门中医药职业学院2023年度院级科学研究项目([科]JMZYYKY20232003)
广东省教育厅重点平台项目(2022GCZX023)。
关键词
簕菜
提取物
含量
体外抗氧化活性
Acanthopanax trifoliatus
extract
content
in vitro antioxidant activity
