摘要
为了评价五倍子颗粒对靶动物蛋鸡的安全性,在3组海兰褐蛋鸡中分别饲喂1、3或5倍推荐剂量的五倍子颗粒,同时设置空白对照组,观察蛋鸡的生产性能变化,并测定蛋品质及血液生理生化指标,测定脏器指数和肠道通透性,观察病理组织学变化。结果显示,试验各组临床体征均正常,与空白对照组相比,体重、生产性能、蛋品质、血液生理生化指标及脏器指数均无显著性差异,剖检以及组织显微结构未发现病变;肠道通透性检测显示,高剂量组空肠上皮紧密连接蛋白基因(CLDN1)和回肠上皮闭合蛋白基因(OCLN)表达显著上调(P<0.05);中剂量组回肠上皮紧密连接蛋白基因(CLDN1)和闭合蛋白基因(OCLN)表达显著上调(P<0.05)。上述结果表明,五倍子颗粒在0.5 g/kg剂量以下使用是安全的,且具有强化肠道屏障的作用。
To evaluate the safety of Galla chinensis granules in laying hens(target animals),3 groups Hailan brown laying hens were fed by 1,3,or 5 times clinically recommended dose of Galla chinensis granules,and a blank control group was set up.The changes in production performance were recorded,egg quality,blood physiological and biochemical indexes were determined,organ indexes and intestinal permeability were measured,and histological changes were observed.The results showed that no abnormal changes occurred in the clinical conditions.Compared with the blank control group,there were no significant differences in weight,production performance,egg quality,blood physiological and biochemical indexes,and organ index,and no lesions were found in autopsy and tissue microstructure.Intestinal permeability tests revealed that the expressions of the CLDN1 in the jejunal epithelium and the OCLN in the ileal epithelium were significantly upregulated in the high dose group(P<0.05).Similarly,the medium dose group exhibite significant upregulation of CLDN1 and OCLN in the ileal epithelium(P<0.05).The results indicate that Galla chinensis granules are safe in the dose below 0.5 g/kg and can enhance the intestinal barrier.
作者
李穗湘
茹梦珂
郝宝成
杨珍
刘宇
王玲
王胜义
胡庭俊
崔东安
LI Suixiang;RU Mengke;HAO Baocheng;YANG Zhen;LIU Yu;WANG Ling;WANG Shengyi;HU Tingjun;CUI Dong’an(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs/Key Laboratory of New Animal Drug Project of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)
出处
《中国兽医科学》
北大核心
2025年第10期1399-1406,共8页
Chinese Veterinary Science
基金
国家重点研发计划专项(2022YFD1801102)
国家重点研发计划项目(2023YFD1800802)
中央级公益性科研院所基本科研业务费专项(Y2022YJ14)
兽用天然药物与抗生素替代创新工程项目(25-LZIHPS-03)。
关键词
五倍子颗粒
蛋鸡
安全性评价
单宁酸
Galla chinensis granules
laying hens
safety assessment
tannic acid
