摘要
探讨了氧化三甲胺(TMAO)对血管内皮细胞(HAECs)和血管平滑肌细胞(VSMCs)成骨化的作用机制:以不同浓度的TMAO处理HAECs,检测间质化指标与成骨化指标的变化,并检测培养基中各种细胞因子,如白介素6(IL-6)、白介素8(IL-8)等炎症相关因子的变化;选取增长最明显的细胞因子作为后续研究对象,将其加入VSMCs的培养基中进行培养处理,检测成骨化指标的变化.结果发现,TMAO可促进HAECs发生成骨化(p<0.05),且这种变化与内皮间质转化(End-MT)相关;TMAO可促进HAECs分泌炎症因子IL-8(p<0.05),且此效应可促进VSMCs发生成骨化(p<0.05).由此可得,TMAO可通过End-MT过程促进HAECs发生成骨化,并诱导HAECs分泌IL-8等炎性细胞因子促使VSMC发生成骨化.
This study investigates the mechanism of Trimethylamine Oxide(TMAO)in the ossification of human aortic endothelial cells(HAECs)and vascular smooth muscle cells(VSMCs).After treating HAECs with different concentrations of TMAO,we detect changes in their mesenchymal and osteogenic markers,and measure changes in various cytokines such as interleukin-6(IL-6),interleukin-8(IL-8),and other inflammatory cytokines.Then we select the most significantly elevated cytokine for further experiments,in which it is added to the VSMC culture medium,and subsequent changes in osteogenic markers are detected.Result shows that TMAO can promote the ossification of HAECs(p<0.05),and this change is associated with endothelial-mesenchymal transition(End-MT).Furthermore,TMAO can promote the secretion of inflammatory cytokine IL-8 from HAECs(p<0.05),and this effect can subsequently promote the ossification of VSMCs(p<0.05).We can draw a conclusion that TMAO can promote the ossification in HAECs through the End-MT process and induce the secretion of inflammatory cytokines such as IL-8 to promote VSMC ossification.
作者
陈铭家
周建庆
杨曦
Chen Mingjia;Zhou Jianqing;Yang Xi(Health Science Center,Ningbo University,Ningbo,Zhejiang 315000;Ningbo Medical Center Lihuili Hospital,Ningbo University,Ningbo,Zhejiang 315000)
出处
《嘉兴大学学报》
2025年第6期54-60,共7页
Journal of Jiaxing University
基金
宁波市自然科学基金项目(2023J217)。
关键词
氧化三甲胺
血管内皮细胞
血管平滑肌细胞
成骨化
血管钙化
trimethylamine oxide
endothelial cells
vascular smooth muscle cells
ossification
vascular calcification
