摘要
本文通过体外孵育实验和体内灌喂实验,旨在探究乙酸钠(Sodium acetate,SA)对鲤(Cyprinus carpio)肠道糖吸收的作用。实验中,将不同浓度的SA与鲤肠道组织共孵育,并对鲤进行灌喂处理。实验结束后,检测肠道组织的葡萄糖含量以及葡萄糖转运载体(sglt1、glut2)、肠道屏障基因(zo-1、claudin)和信号通路关键基因的表达量。实验结果显示,SA显著提高了肠道中葡萄糖的含量,增强了糖转运载体基因(sglt1、glut2)和紧密连接蛋白基因(zo-1、claudin)的表达。此外,SA可以通过激活AMPK信号通路促进鲤肠道的糖吸收并增强肠道屏障功能,且研究发现SA调控鲤肠道糖吸收的最适浓度为8 mM。综上,SA可以通过调控糖转运载体、能量代谢及紧密连接蛋白基因的表达,有效提高鲤肠道的糖吸收功能。
This study aimed to investigate the effect of sodium acetate(SA)on intestinal glucose absorption in Cyprinus carpio through in vitro incubation experiments and in vivo feeding experiments.In the experiments,different concentrations of SA were co-incubated with common carp intestinal tissues,and common carp were fed with SA.Afier the experiments,the glucose content in the intestinal tissues and the expression levels of glucose transporter genes(sglt1,glut2),intestinal barrier genes(zo-1,claudin),and key genes in the signaling pathways were detected.The experimental results showed that SA significantly increased the glucose content in the intestine,enhanced the expression of glucose transporter genes(sglt1,glut2)and tight junction protein genes(zo-1,claudin).In addition,SA could promote intestinal glucose absorption and enhance intestinal barrier function in common carp by activating the AMPK signaling pathway.Moreover,the optimal concentration of SA for regulating intestinal glucose absorption in common carp was found to be 8 mM.In conclusion,SA can effectively improve intestinal glucose absorption in common carp by regulating the expression of glucose transporters,energy metabolism,and tight junction proteins.
作者
朱书贤
李晓冉
冯军厂
Zhu Shuxian;Li Xiaoran;Feng Junchang(Engineering Technology Research Center for Aquatic Animal Cultivation of Henan Province/College of Fisheries,Henan Normal University,Xinxiang,Henan 453007)
出处
《河南水产》
2025年第5期27-31,共5页
HENAN FISHERIES
基金
河南省重点科技攻关项目(242102110048)
河南省自然科学基金面上项目(252300420199)。
关键词
鲤
乙酸钠
肠道糖吸收
肠道屏障
Cyprinus carpio
sodium acetate
intestinal glucose absorption
intestinal barrier
