摘要
为揭示斜带石斑鱼对碳水化合物利用的特点，研究了3个碳水化合物水平及饥饿处理对其生长、血浆生化指标及肝／肌糖原的影响。实验选取300尾初重为（35±0．28）g的幼鱼，设置持续投喂高（35％，C H）、中（21％，C M）、低（7％，C L）3个碳水化合物水平组，以及持续投喂组（C M）、饥饿再投喂组（R，饥饿4周＋投喂4周，投喂C。组饲料）、饥饿组（S）3个投喂模式组。饲养8周后饥饿24h，以30mg／100g体质量腹腔注射葡萄糖研究其代谢反应。结果显示，增重率和特定生长率随饲料碳水化合物水平的增加而升高，但无显著差异，饲料系数以C L组最高。不同碳水化合物水平下，各组注射葡萄糖后1—3h血糖水平达峰值，但C M组在6h内迅速回到注射前水平；各组血浆胰岛素水平均先降后升，但C L，组在3h后急剧下降；各组肝糖原、血浆甘油三酯含量在注射后1h内均显著上升。饥饿处理下，持续投喂组（C M）血糖水平在6h时迅速恢复至注射前水平；C M组和R组血浆胰岛素在1h内显著下降，但S组持续上升；C M组血浆甘油三酯含量在0—6h内显著高于其他两组。研究表明，饲喂中等水平碳水化合物（21％）比较符合斜带石斑鱼糖耐受能力，而饥饿处理下则以持续投喂组糖耐受能力最强。
The experiment was conducted to determine the effect of dietary carbohydrate levels （CBH） and starvation on the growth performance, biochemical indices in plasma and liver/muscle glycogen content of the grouper. Total 300 fish were randomly distributed into 5 treatments with triplicate groups of 20 fish （initial weight ：35 ± 0.28 g）in each treatment. Three isonitrogenous and isoenergetic diets were formulated containing three carbohydrate levels （ 35 % CH, 21% CM , 7 % CL ）, combined with starvation group （ S ） and refeeding group（R, starving 4W ＋ refeeding 4W,21% carbohydrate in the diet）. Experiment lasted 8W. 14 fish in each treatment were selected and injected with 300 mg glucose per 100 g body weight for glucose tolerance test（GTT） after the end of experiment and blood sampling at 0,1,3,6,12,24,48 h. The results showed that the weight gain and specific growth rate increased with dietary carbohydrate level increase but no significant difference（P 〉0.05） ,and the feed coefficient rate of CL group was the highest（P 〈 0.05 ）. Plasma glucose levels significantly increased in 1 -3 h and then decreased in CH, CM and CL （P 〈 0.05 ）. Plasma glucose levels in CM returned to the initial values within 6 h after being injected and initial level of plasma glucose content in CH and CL were obtained within 12 h. Plasma insulin content in CH, CM and CL decreased at 1 h and then increased,but insulin level of CL group significantly decreased at 3 h（P 〈 0.05）. Glycogen content in liver and plasma triglyceride levels were significantly increased in CH,CM and CLwithin 1 h after injection （ P 〈 0.05 ）. CM, S and R groups presented persistent hyperglycemia after being injected and CM restored initial level quickly within 6 h. Plasma insulin levels of CM and R groups significantly decreased within 1 h（ P 〈 0.05 ） ,but that of R group continued to rise within 12 h. Plasma triglyceride levels of CM were higher than those of S and R groups within 0 to 6 h（ P 〈 0.05 ）. The results of GTT indicate that proper carbohydrate level in diet（21% ）and continuous feeding can significantly improve glucose tolerance of the grouper.
出处
《水产学报》
CAS
CSCD
北大核心
2014年第4期550-559,共10页
Journal of Fisheries of China
基金
国家自然科学基金(31272673)
公益性行业(农业)科研专项(201003020)
广东省珠江学者基金(GDUPS 2011)
广东省高等学校科技创新重点项目(2011)
广东省自然科学基金(S2013010016511)
关键词
斜带石斑鱼
碳水化合物
营养状态
葡萄糖
糖耐量
Epinephelus coioides
carbohydrate
nutrition status
glucose
glucose tolerance test
