摘要
[目的]探究外源性谷胱甘肽对老龄小鼠体内抗氧化能力的改善作用。[方法]选取老龄小鼠作为研究对象,连续30 d灌胃不同剂量的谷胱甘肽(166.7,83.3,41.7 mg/kg),测定小鼠血清和肝组织匀浆中丙二醛(MDA)、8-异构前列腺素、蛋白质羰基(PCO)和还原型谷胱甘肽含量,以及总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化酶(GSH-PX)活力。[结果]与空白对照组相比,外源性谷胱甘肽高剂量组能够显著降低老龄小鼠体内MDA和PCO含量,提高小鼠血清中T-SOD活力,提高老龄小鼠体内还原型谷胱甘肽含量(P<0.05),且受试物对老龄小鼠体重增长及脏器指数无不良影响。[结论]谷胱甘肽对机体衰老过程中的氧化应激状态具有良好的改善作用。
[Objective]To investigate the effect of exogenous glutathione(GSH)on improving antioxidant capacity in aging mice.[Methods]Aging mice were administered GSH by gavage at different doses(166.7,83.3,and 41.7 mg/kg)continuously for 30 days.Levels of malondialdehyde(MDA),8-isoprostane,protein carbonyl(PCO),and reduced GSH in serum and liver homogenate,as well as activities of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-PX),were measured.[Results]Compared with the blank control group,the high-dose GSH group showed significant reductions in MDA and PCO levels,increased T-SOD activity in serum,and elevated reduced GSH content in aging mice(P<0.05).No adverse effects on body weight gain or organ indices were observed.[Conclusion]GSH effectively improves oxidative stress status during the aging process.
作者
汪明
余永名
王宇慧
张帅佳
罗瑶
马超
WANG Ming;YU Yongming;WANG Yuhui;ZHANG Shuaijia;LUO Yao;MA Chao(Anhui Zhengxing Biopharmaceutical Technology Co.,Ltd.,Xuancheng,Anhui 242000,China;College of Biological Science and Technology,Beijing Forestry University,Beijing 100083,China;Beijing Key Laboratory of Forest Food Processing and Safety,Beijing 100083,China)
出处
《食品与机械》
北大核心
2025年第10期126-131,共6页
Food and Machinery
基金
国家自然科学基金项目(编号:31770614)。
关键词
谷胱甘肽
老龄小鼠
活性氧
抗氧化能力
glutathione
aging mice
reactive oxygen species
antioxidant activity
