摘要
目的:探究谷胱甘肽酵母对环磷酰胺诱导的免疫低下小鼠的免疫调节作用。方法:通过腹腔注射环磷酰胺建立免疫低下小鼠模型,分别灌胃谷胱甘肽酵母低(128.45 mg/kg·bw)、中(1284.5 mg/kg·bw)、高1(2500 mg/kg·bw)剂量,观察小鼠的生长状态,测定小鼠体质量、腹腔巨噬细胞功能、免疫器官系数、血浆细胞因子水平、血浆中谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)的表达、免疫球蛋白含量,评价小鼠脾脏和回肠组织病理变化,并对脾脏进行免疫组织化学分析,以此探究谷胱甘肽酵母对免疫功能的影响。结果:谷胱甘肽酵母可以显著提高免疫低下小鼠的巨噬细胞吞噬功能、脾脏系数及胸腺系数(P<0.05),血浆GSH含量、GSH-Px活性分别提高22.84%,29.42%,血浆干扰素γ(IFN-γ)、免疫球蛋白IgA、IgG含量分别上升341.90%,25.40%,55.69%。病理组织切片结果显示,谷胱甘肽酵母能有效改善环磷酰胺造成的脾脏和回肠组织病理损伤,免疫组织化学分析结果显示谷胱甘肽酵母显著提高脾脏细胞表面CD4+表达,降低CD8+表达(P<0.05)。结论:谷胱甘肽酵母对免疫低下小鼠的免疫调节功能具有改善作用。
Objective:To investigate the immunomodulatory effect of glutathione yeast on cyclophosphamide-induced immunocompromised mice.Methods:A mouse model of immunocompromise was established by intraperitoneal injection of cyclophosphamide.Mice were intragastrically administered with glutathione yeast at low(128.45 mg/kg·bw),medium(1284.5 mg/kg·bw),or high(2500 mg/kg·bw)doses.The growth status of mice was observed,and body weight,peritoneal macrophage function,immune organ indices,plasma cytokine levels,plasma glutathione(GSH)content,glutathione peroxidase(GSH-Px)activity,and immunoglobulin concentrations were determined.Pathological changes in the spleen and ileum were evaluated,and immunohistochemical analysis of the spleen was performed to explore the effect of glutathione yeast on immune function.Results:Glutathione yeast significantly enhanced the phagocytic function of macrophages,as well as the spleen and thymus indices in immunocompromised mice(P<0.05).Compared with the model group,plasma GSH content and GSH-Px activity increased by 22.84%and 29.42%,respectively;plasma IFN-γ,IgA,and IgG levels increased by 341.90%,25.40%,and 55.69%,respectively.Pathological sections showed that glutathione yeast effectively ameliorated cyclophosphamide-induced pathological damage to the spleen and ileum.Immunohistochemical analysis revealed that glutathione yeast significantly upregulated CD4+expression and downregulated CD8+expression on splenic cells(P<0.05).Conclusion:Glutathione yeast exerts a beneficial immunomodulatory effect on immunocompromised mice.
作者
王虎
袁雪梅
余海立
刘晓雯
张彦
WANG Hu;YUAN Xuemei;YU Haili;LIU Xiaowen;ZHANG Yan(National Key Laboratory of Agricultural Microbial Resources Exploration and Utilization,Yichang 443000,Hubei;Hubei Provincial Key Laboratory of Yeast Function,Yichang 443000,Hubei;Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy&College of Basic Medical Sciences,Medical College,China Three Gorges University,Yichang 443002,Hubei)
出处
《中国食品学报》
北大核心
2025年第11期155-163,共9页
Journal of Chinese Institute of Food Science and Technology
基金
国家重点研发计划项目(2022YFF1103305)
湖北省自然科学基金宜昌创新联合基金项目(2024AFD180)。
关键词
谷胱甘肽酵母
免疫
环磷酰胺
glutathione yeast
immunity
cyclophosphamide
