摘要
目的评估β-烟酰胺单核苷酸(β-nicotinamide mononucleotide,NMN)对环磷酰胺(cyclophosphamide,CTX)诱导的免疫抑制小鼠免疫功能的影响。方法将ICR小鼠随机分为空白组、模型组、阳性药组(盐酸左旋咪唑)和NMN低、中、高剂量组。除空白组外,其余组均通过腹腔注射CTX建立免疫抑制模型。给药组小鼠每天灌胃给予相应药物。通过检测免疫功能低下的小鼠体重、免疫器官指数、细胞免疫功能、体液免疫功能、单核巨噬细胞功能和NK细胞活性,评估NMN对免疫抑制小鼠免疫功能的影响。此外,检测免疫功能低下小鼠血清和肝脏组织中炎症因子水平和肝脏氧化应激指标的表达水平。结果NMN能改善免疫抑制小鼠的体重和免疫器官指数。在免疫功能改善方面,NMN对小鼠脾淋巴细胞增殖无影响;中、高剂量组的NMN均极显著增强了免疫抑制小鼠的细胞免疫功能、体液免疫功能及NK细胞活性(P<0.01);3个剂量组的NMN均显著或极显著改善了单核巨噬细胞功能(P<0.05或P<0.01)。此外,NMN提高了CTX处理小鼠肝脏的抗氧化能力,表现为显著或极显著降低肝脏中丙二醛(malondialdehyde,MDA)含量(P<0.05或P<0.01)并极显著增加过氧化氢酶(catalase,CAT)活性(P<0.01)。除低剂量组外,其他剂量组NMN还极显著提高了超氧化物歧化酶(superoxide dismutase,SOD)含量。除低剂量组外,NMN极显著增加了血清中白细胞介素-2(interleukin-2,IL-2)和白细胞介素-6(interleukin-6,IL-6)水平(P<0.01),而3个剂量组NMN均极显著提高了肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)水平(P<0.01),表明其能调节炎症反应。结论本研究首次在细胞免疫、体液免疫和非特异性免疫3个层面系统评估了NMN的改善免疫功能的作用。研究结果证实了NMN是一种潜在的免疫调节剂,可能通过多种机制增强免疫功能,改善CTX诱导的免疫抑制状态,有望成为治疗免疫相关疾病的潜在方法。
Objective To evaluate the effect ofβ-nicotinamide mononucleotide(NMN)on the immune function in mice with immunosuppression induced by cyclophosphamide(CTX).Methods ICR mice were randomly divided into a blank group,a model group,a positive drug group(levamisole hydrochloride),and low,medium,and high-dose NMN groups.Except for the blank group,immunosuppression models were established in the other groups by intraperitoneal injection of CTX.Mice in the treatment groups were administered corresponding drugs by gavage daily.The effects of NMN on the immune function of immunosuppressed mice were evaluated by detecting body weight,immune organ index,cellular immune function,humoral immune function,monocyte-macrophage function and NK cell activity in mice with impaired immune function.Additionally,the levels of inflammatory factors in serum and liver tissues and the expression levels of liver oxidative stress indicators in immunocompromised mice were measured.Results NMN improved the body weight and immune organ indices in immunosuppressed mice.Regarding immune function improvement,NMN had no effect on splenic lymphocyte proliferation in mice.The medium-and high-dose groups of NMN significantly enhanced cellular immune function,humoral immune function,and NK cell activity in immunosuppressed mice(P<0.01).All three dose groups of NMN significantly or extremely significantly improved mononuclear macrophage function(P<0.05 or P<0.01).Additionally,NMN enhanced the antioxidant capacity of the liver in CTX-treated mice,as evidenced by significantly or extremely significantly reduced malondialdehyde(MDA)content(P<0.05 or P<0.01)and extremely significantly increased catalase(CAT)activity(P<0.01).Except for the low-dose group,the other NMN dose groups also extremely significantly increased superoxide dismutase(SOD)content.Except for the low-dose group,NMN extremely significantly increased serum levels of interleukin-2(IL-2)and interleukin-6(IL-6)(P<0.01),while all three dose groups of NMN extremely significantly elevated tumor necrosis factor-alpha(TNF-α)levels(P<0.01),indicating its ability to regulate inflammatory responses.Conclusion This study presents the first systematic evaluation of NMN’s effects on enhancing immune function,comprehensively examining cellular,humoral,and non-specific immunity.The research results confirmed that NMN is a potential immunomodulator that may enhance immune function through multiple mechanisms,improve the immunosuppressed state induced by CTX,and potentially serve as a potential method for treating immune-related diseases.
作者
刘晶晶
刘树森
乔菲
许玮仪
刘静
徐蓓蕾
LIU Jing-Jing;LIU Shu-Sen;QIAO Fei;XU Wei-Yi;LIU Jing;XU Bei-Lei(National Institutes for Food and Drug Control,Beijing 100050,China;School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)
出处
《食品安全质量检测学报》
2025年第21期294-302,共9页
Journal of Food Safety and Quality
关键词
β-烟酰胺单核苷酸
免疫抑制
细胞免疫
体液免疫
非特异性免疫
β-nicotinamide mononucleotide
immunosuppression
cellular immunity
humoral immunity
non-specific immunity
