摘要
该文对蝉蛹肽(Cryptotympana atrata Peptide,CA-Peptide)的制备工艺进行优化,并比较CA-Peptide和蝉蛹蛋白(Cryptotympana atrata Protein,CA-Protein)的免疫调节活性。最佳工艺:碱性蛋白酶37071和胰蛋白酶复合酶解,加酶量各4%(m/m),底物质量分数5%(m/m)、初始pH值10.5、酶解温度50℃,两种蛋白酶先后酶解1.5 h。动物试验显示,CA-Peptide和CA-Protein均能不同程度改善环磷酰胺所致免疫低下小鼠的相关指标。相同剂量下,CA-Peptide组小鼠的脾脏指数和脾淋巴细胞增殖率分别为2.40 mg/g和51.07%,显著(P<0.05)高于CA-Protein组;CA-Peptide组小鼠的血清免疫球蛋白G、免疫球蛋白A、免疫球蛋白M、肿瘤坏死因子α、白细胞介素-4和白细胞介素-10含量分别为701.67、299.19、239.88、20.11、33.86和71.15 ng/L,显著(P<0.05)高于CA-Protein组;CA-Peptide组小鼠的胸腺指数和血清γ干扰素含量分别为1.36 mg/g和36.26 ng/L,显著(P<0.05)低于CA-Protein组;CA-Peptide组小鼠的自然杀伤细胞活性及血清白细胞介素-2含量与CA-Protein组无显著性差异(P>0.05)。研究表明,酶解工艺显著改变了CA-Protein的免疫调节活性,产物CA-Peptide对多项免疫指标的改善作用优于CA-Protein。
The preparation process of Cryptotympana atrata peptide(CA-peptide)was optimized,and the immunomodulatory activities of CA-peptide and C.atrata protein(CA-protein)were compared.The optimal preparation conditions were determined as follows:4%(m/m)alkaline proteinase 37071,4%(m/m)trypsin,5%(m/m)substrate,an initial pH of 10.5,and an enzymolysis temperature of 50℃.Enzymolysis was carried out separately with each protease for 1.5 h.Animal experiments showed that the related indices in immunocompromised mice induced by cyclophosphamide were improved to varying degrees by CA-peptide and CA-protein.At the same dose,the spleen index and proliferation rate of splenic lymphocytes in the CA-peptide group(2.40 mg/g and 51.07%,respectively)were significantly(P<0.05)higher than those in the CA-protein group.The serum immunoglobulin G,immunoglobulin A,immunoglobulin M,tumor necrosis factor-α,interleukin-4,and interleukin-10 levels in the CA-peptide group(701.67,299.19,239.88,20.11,33.86,and 71.15 ng/L,respectively)were significantly(P<0.05)higher than those in the CA-protein group.The thymus index and serum interferon-γlevel in the CA-peptide group(1.36 mg/g and 36.26 ng/L,respectively)were significantly(P<0.05)lower than those in the CA-protein group.There was no significant difference in natural killer cell activity and serum interleukin-2 level between the CA-peptide and CA-protein groups(P>0.05).In conclusion,the enzymatic hydrolysis process has a profound impact on the immunomodulatory activity of CA-protein.Collectively,CA-peptide,exhibited stronger improvement effects on various immune indices compared with CA-protein.
作者
孙明哲
孙亚琴
闫琳
徐淑妹
张宇航
王朝阳
李晓燕
SUN Mingzhe;SUN Yaqin;YAN Lin;XU Shumei;ZHANG Yuhang;WANG Zhaoyang;LI Xiaoyan(Shijiazhuang Yiling Pharmaceutical Co.Ltd.,State Key Laboratory for Innovation and Transformation of Luobing Theory,Shijiazhuang 050035,China;College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083,China)
出处
《现代食品科技》
北大核心
2025年第9期1-10,共10页
Modern Food Science and Technology
基金
国家重点研发计划重点专项(2023YFC3504300)。
关键词
蝉蛹
肽
免疫调节
酶解
Cryptotympana atrata
peptide
immunomodulation
enzymolysis
