摘要
目的研究特应性皮炎严重程度与皮肤微生物组结构和功能的关系。方法选取2021年7月—2024年7月于本院接受治疗的130例特应性皮炎患者,将患者典型受累皮肤的微生物标本纳入观察组,前臂曲侧未受累皮肤区标本纳入对照组,对比两组的微生物菌群变化及α多样性。另外,根据特应性皮炎积分指数(SCORAD)将患者分为轻度组(46例)、中度组(60例)、重度组(24例),分析三组患者的皮肤微生物菌群α多样性。采用Pearson法分析皮肤微生物菌群α多样性与特应性皮炎患者病情严重程度的相关性。采用京都基因与基因组百科全书(KEGG)预测皮肤微生物的功能通路。结果观察组与对照组共同含有625个操作分类单位(OTU),其中观察组总共有987个OTU,独有362个OTU;对照组总共有686个OTU,独有61个OTU。与对照组相比,观察组的假单胞菌门的相对丰度明显降低(t=18.96,P<0.05),而芽孢杆菌门的相对丰度明显升高(t=21.32,P<0.05)。相较于对照组,观察组的ace指数、chao指数、simpson指数更高,而shannon指数更低(P<0.001)。轻度组的ace指数、chao指数、simpson指数均低于中度组,而shannon指数高于中度组;中度组的ace指数、chao指数、simpson指数均低于重度组,而shannon指数高于重度组(P<0.05)。Pearson相关性分析显示,特应性皮炎患者的ace指数、chao指数、shannon指数、simpson指数与病情严重程度具有明显相关性(P<0.05)。KEGG通路分析显示,特应性皮炎患者的皮肤微生物功能基因主要包括碳水化合物代谢、氨基酸代谢等。结论特应性皮炎患者的皮肤微生物菌群多样性与正常皮肤存在一定差异,且皮肤微生物菌群变化与患者的病情进展密切相关。
Objective To investigate the relationship between the severity of atopic dermatitis,and the composition and function of the skin microbiome.Methods A total of 130 patients with atopic dermatitis who were treated in our hospital from July 2021 to July 2024 were selected.The microbial specimens from the lesion-involved area were included in the observation group,while the specimens from the unaffected skin area of the flexor of the forearm served as the controls.The changes in microbial flora andαdiversity were compared between the two groups.Additionally,the patients were divided into mild group(46 cases),moderate group(60 cases),and severe group(24 cases)based on the Atopic Dermatitis Score Index(SCORAD),and theαdiversity of the skin microbial flora of the three groups was analyzed.The Pearson correlation was used to analyze the correlation between theαdiversity of the skin microbial flora and the severity of the atopic dermatitis.The Kyoto Encyclopedia of Genes and Genomes(KEGG)was used to predict the functional pathways of skin microorganisms.Results The observation group and the control group shared a total of 625 operational taxonomic units(OTUs).The observation group had a total of 987 OTUs,with 362 being unique,while the control group had a total of 686 OTUs,with 61 being unique.Compared with the control group,the relative abundance of the Pseudomonadales in the observation group was significantly lower(t=18.96,P<0.05),while the relative abundance of the Bacillales was significantly higher(t=21.32,P<0.05).Compared with the control group,the ace index,chao index,and simpson index were higher,while the shannon index was lower in the observation group(P<0.001).The ace index,chao index,and simpson index of the mild group were lower than those of the moderate group,while the shannon index was higher than that of the moderate group.The ace index,chao index,and simpson index of the moderate group were lower than those of the severe group,while the shannon index was higher than that of the severe group(P<0.05).Pearson correlation analysis showed that the ace index,chao index,shannon index,and simpson index were significantly correlated with the severity of the disease(P<0.05).KEGG pathway analysis showed that the skin microbial functional genes in patients with atopic dermatitis were mainly related to carbohydrate metabolism and amino acid metabolism.Conclusion The diversity of the skin microbiota in atopic dermatitis-involved area differs from that in the uninvolved skin area,and the changes in the skin microbiota are associated with the disease progression.
作者
周会杰
张大雷
ZHOU Huijie;ZHANG Dalei(First People′s Hospital of Puyang,Anyang 455000,China)
出处
《皮肤性病诊疗学杂志》
2025年第9期651-658,共8页
Journal of Diagnosis and Therapy on Dermato-venereology
基金
2023年度河南省医学科技攻关计划联合共建项目(LHGJ20230914)。
