摘要
本试验旨在探究滩羊肠道菌群和血浆代谢物与平均日增重(ADG)的相关性。选择156只体重相近的滩羊公羔,采用颈夹法饲喂75 d,其中过渡期15 d,预试期10 d,正试期50 d。试验期结束后,将ADG>平均值+0.5倍标准差的试验羊分为高ADG组(HADG组),ADG<平均值-0.5倍标准差的试验羊分为低ADG组(LADG组),比较2组滩羊(各挑选5只)的肠道菌群和血浆代谢物差异,并进行相关性分析。结果表明:1)在属水平上,HADG组盲肠中大肠杆菌属(Colidextribacter)、产己酸菌属(Caproiciproducens)、norank_f_norank_o_Veillonellales-Selenomonadales、普拉梭菌属(Faecalibacterium)、肠杆菌属(Enterorhabdus)相对丰度显著高于LADG组(P<0.05),脱硫弧菌属(Desulfovibrio)相对丰度显著低于LADG组(P<0.05);HADG组直肠中拟普雷沃氏菌属(Alloprevotella)、沼泽微杆菌属(Paludicola)相对丰度显著高于LADG组(P<0.05),厌氧支原体属(Anaeroplasma)、普拉梭菌属(Faecalitalea)相对丰度显著低于LADG组(P<0.05)。2)血浆代谢组学分析共筛选到104种差异代谢物,与HADG相比,LADG组中有53种代谢物下调,51种代谢物上调。这些代谢物在赖氨酸生物合成、苯丙氨酸代谢、糖酵解/糖异生代谢通路显著富集(P<0.05)。对血浆差异代谢物C20硫苷(C20 sulfatide)、棕榈油酸(palmitoleic acid)、L-乳酸(L-lactic acid)、氢化肉桂酸(hydrocinnamic acid)和L-赖氨酸(L-lysine)与肠道差异菌群进行相关性分析,结果显示盲肠脱硫弧菌属与L-乳酸和棕榈油酸呈显著或极显著正相关(P<0.05或P<0.01),norank_f_norank_o_Veillonellales-Selenomonadales与L-乳酸呈显著负相关(P<0.05);直肠厌氧支原体属与L-乳酸、C20硫苷和棕榈油酸呈显著正相关(P<0.05);直肠普拉梭菌属与L-乳酸、C20硫苷呈显著或极显著正相关(P<0.05或P<0.01),与L-赖氨酸呈显著负相关(P<0.05)。综上所述,滩羊肠道菌群通过血浆代谢物影响滩羊ADG。
This experiment was conducted to study the correlation analysis of gut microbiota and plasma metabolites with average daily gain(ADG)of Tan sheep.A total of 156 male Tan lambs with similar body weights were selected and fed using the neck clamp method for 75 days,including a transition period of 15 days,a pre-test period of 10 days,and a trial period of 50 days.After the end of the trial period,the sheep with ADG>mean+0.5 times standard deviation was divided into the high ADG group(HADG group),and the sheep with ADG<mean+0.5 times standard deviation was divided into the low ADG group(LADG group),compared the differences of gut microbiota and plasma metabolites of Tan sheep between two groups(5 sheep in each group),and conducted the correlation analysis.The results showed as follows:1)at the genus level,the relative abundances of Colidextribacter,Caproiciproducens,norank_f_norank_o_Veillonellales-Selenomonadales,Faecalibacterium and Enterorhabdus in cecum of HADG group were significantly higher than those of LADG group(P<0.05),and the Desulfovibrio relative abundance was significantly lower than that of LADG group(P<0.05);the relative abundances of Alloprevotella and Paludicola in rectum of HADG group were significantly higher than those of LADG group(P<0.05),and the Anaeroplasma and Faecalitalea relative abundances were significantly lower than those of LADG group(P<0.05).2)The plasma metabolomics analysis identified 104 differential metabolites,compared to HADG group,53 metabolites were down-regulated and 51 metabolites were up-regulated in LADG group.These metabolites were significantly enriched in pathways such as lysine biosynthesis,phenylalanine metabolism and glycolysis/gluconeogenesis(P<0.05).The plasma differential metabolites such as C20 sulfatide,palmitoleic acid,L-lactic acid,hydrocinnamic acid,and L-lysine were selected for correlation analysis with gut differential microbiota,the results showed that Desulfovibrio in cecum was significantly positively correlated with L-lactic acid and palmitoleic acid(P<0.05 or P<0.01),and norank_f_norank_o_Veillonellales-Selenomonadales was significantly negatively correlated with L-lactic acid(P<0.05);the Anaeroplasma in rectum was significantly positively correlated with L-lactic acid,C20 sulfatide and palmitoleic acid(P<0.05);the Faecalibacterium in rectum was significantly positively correlated with L-lactic acid and C20 sulfatide(P<0.05 or P<0.01),and significantly negatively correlated with L-lysine(P<0.05).In conclusion,the gut microbiota of Tan sheep influences ADG through plasma metabolites.
作者
郑浩
张馨蕊
施安
马俊
张驹
杨冲
刘统高
吴爽
王倩
陶金忠
ZHENG Hao;ZHANG Xinrui;SHI An;MA Jun;ZHANG Ju;YANG Chong;LIU Tonggao;WU Shuang;WANG Qian;TAO Jinzhong(College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;College of Food Science and Engineering,Northwest A&F University,Yangling 712021,China;Animal Husbandry WorkStation of Ningxia Hui Autonomous Region,Yinchuan 750021,China)
出处
《动物营养学报》
北大核心
2025年第9期6267-6280,共14页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
盐池滩羊种质特征以及优良性状形成机制的研究(U21A201210)。
