摘要
为探究海水总氮分析法[碱性过硫酸钾氧化法(GB17378.4—2007)]和水质总氮分析法[碱性过硫酸钾氧化-紫外分光光度法(HJ636—2012)]适用盐度范围以及水质总氮分析法无法检验高盐度样品的原因,通过比较盐度0~35内,2种水质分析法,8个不同的标准曲线的空白值、r^(2)以及硝酸盐还原率、标准曲线的差异,确定2种总氮分析法的适用盐度范围。试验结果表明:海水总氮分析法可以检测不同盐度梯度的海水样品,回收率为92%~100%;水质总氮分析法仅可检测盐度≤20的海水样品,其回收率为92%~106%,当盐度>20时,使用水质总氮分析法的样品中存在过硫酸钾残留。后续通过过硫酸钾残留试验和醋酸滴定试验比较不同盐度下水质总氮分析法的过硫酸钾残留浓度,验证过硫酸钾氧化法不可测定高盐度的原因。最后比较海水总氮分析法与水质总氮分析法检测海水样品,确定稀释法能否检测海水样品。另外,水质总氮分析法无法检测高盐度海水稀释样品。本试验系统地分析了2种总氮分析方法的适用盐度范围,解释了水质总氮分析法不可检测高盐度样品的原因,为今后总氮指标检测与方法改良提供参考和依据。
This study systematically explored the applicable salinity range of seawater total nitrogen analysis method[alkaline potassium persulfate oxidation method(GB17378.4-2007)]e from 92%to 100%.The total nitrogen analysis method can only detect seawater samples with salinity≤20,with the recovery rate from 92%to 106%.At the salinity of ovef^(2)0,there is potassium persulfate residue in the sample using the water quality total nitrogen analysis method.Subsequently,the potassium persulfate residual concentration of the total nitrogen analysis method of water quality at different salinities was compared by the potassium persulfate residual experiment and acetic acid titration experiment to verify why the potassium persulfate oxidation method could not determine high salinity.Finally,the total nitrogen analysis method of seawater and the total nitrogen analysis method of water quality are compared to detect seawater samples to determine whether the dilution method can detect seawater samples.In addition,the water quality total nitrogen analysis method cannot detect high-salinity seawater diluted samples.The finding systematically analyzes the applicable salinity range of the two total nitrogen analysis methods,explains why the water quality total nitrogen analysis method can not detect high-salinity samples,and provides a reference and basis for the detection of total nitrogen index and method improvement in the future.
作者
尹智践
姜程程
刘健龙
王世伟
王连顺
丛玉婷
王丽
王华
王媛
卢亚楠
杨国军
YIN Zhijian;JIANG Chengcheng;LIU Jianlong;WANG Shiwei;WANG Lianshun;CONG Yuting;WANG Li;WANG Hua;WANG Yuan;LU Yanan;YANG Guojun(National Demonstration Center for Experimental Aquaculture Education,Ministry of Education,College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Environment Controlled Aquaculture(Dalian Ocean University),Ministry of Education,Dalian 116023,China;Dalian Key Laboratory of Breeding,Reproduction and Aquaculture of Crustaceans,Dalian 116023,China)
出处
《水产科学》
北大核心
2025年第5期829-835,共7页
Fisheries Science
基金
大连市青年科技之星项目(2021RQ092)
辽宁省海洋经济发展专项资金项目(1638837263397)
辽宁省教育厅面上项目(LJKZ0706
LJKMZ20221104)
辽宁省科技厅博士科研启动基金计划项目(2022-BS-278)
大连海洋大学博士科研启动基金计划项目(110222206)
设施渔业教育部重点实验室(大连海洋大学)开放课题项目(2021-MOEKLECA-KF-02202223)
大连海洋大学教改项目(2023-59-2-6).
关键词
水产养殖
盐度
总氮
检测方法
aquaculture
salinity
total nitrogen
detection method
