摘要
目的探究重庆市食源性伦敦沙门氏菌的耐药及基因组特征。方法对2022—2024年重庆市分离的27株食源性伦敦沙门进行鉴定、全基因组测序及药敏分析,并开展全基因组单核苷酸多态性分子溯源、耐药及毒力预测。结果27株沙门氏菌中耐药菌株占比66.67%,对氨苄西林的耐药率高达59.26%,对甲氧苄啶/磺胺甲恶唑、四环素、氯霉素、庆大霉素、阿奇霉素的耐药率均达到40%以上,多重耐药菌株占比51.85%。系统发育分析发现,食品株和病例株存在一定的聚类差异,所有食品分离株均与国内参考菌株聚类,而9株病例株中,5株与国内食品株及参考株聚类,另外4株与国外参考菌株聚类,与其余分离株存在明显的遗传差异。27株沙门菌携带27个耐药基因,耐药基因预测结果与耐药表型高度一致。结论重庆市食源性伦敦沙门氏菌感染食品类别多样,整体耐药性高,多重耐药性严重,食品分离株和病例株存在聚类差异,提示需警惕国内流动风险和国外输入风险,整合全基因组测序加强沙门氏菌的跨地传播及耐药监测,规范抗生素的使用。本研究为优化区域耐药菌防控策略、遏制食源性传播链提供了科学依据。
Objective To explore the antibiotic resistance,genomic characteristics of foodborne Salmonella London in Chongqing.Methods Identification,whole-genome sequencing and antibiotic resistance analysis were conducted on 27 strains of foodborne Salmonella London isolated in Chongqing from 2022 to 2024.Additionally,molecular tracing based on whole genome single nucleotide polymorphism,as well as predictions of antibiotic resistance and virulence,were performed.Results Among the 27 strains,66.67% were antibiotic-resistant,with a resistance rate to ampicillin reached a staggering 59.26%.Furthermore,the resistance rate to trimethoprim/sulfamethoxazole,tetracycline,chloramphenicol,gentamicin and azithromycin all exceeded 40%,while the proportion of multidrug-resistant strains accounted for 51.85%.Phylogenetic analysis revealed that there were some clustering differences between food strains and diarrhea case strains.All food isolates were clustered with domestic reference strains,while 5 out of 9 clinical isolates clustered with food isolates in the domestic branch,and 4 clustered with foreign reference strains,there were significant genetic differences between them and the remaining strains.The 27 strains carried 27 resistance genes,and the predicted resistance genes were highly consistent with the phenotype.Conclusion Foodborne Salmonella London infections in Chongqing are characterized by diverse contamination sources,elevated antibiotic resistance rates,severe multidrug resistance,and distinct phylogenetic clustering patterns between food isolates and clinical strains.These findings highlight the need for vigilance against both domestic transmission risks and international import risks.Whole-genome sequencing should be integrated to strengthen the monitoring of cross-regional transmission dynamics and antibiotic resistance evolution of Salmonella,and the use of antibiotics should be standardized.This study establishes a scientific basis for refining regional antimicrobial resistance containment strategies and disrupting foodborne transmission chains.
作者
聂术君
刘高敏
赵婷
廖春艳
王玲
段刚
孔令汉
李志峰
王文斟
NIE Shu-Jun;LIU Gao-Min;ZHAO Ting;LIAO Chun-Yan;WANG Ling;DUAN Gang;KONG Ling-Han;LI Zhi-Feng;WANG Wen-Zhen(Chongqing Center for Disease Control and Prevention(Chongqing Academy of Preventive Medicine),Chongqing 400707,China;Chongqing Key Laboratory of Highly Pathogenic Microbes,Chongqing 400707,China;Chongqing Research Center for Disease Prevention and Public Health,Chongqing 400707,China;Chongqing Wanzhou District Center for Disease Control and Prevention,Chongqing 404100,China)
出处
《食品安全质量检测学报》
2025年第18期108-119,共12页
Journal of Food Safety and Quality
基金
重庆市医学科研项目(卫生健康委员会和科技局联合)(2024QNXM056)
重庆市自然科学基金项目(CSTB2022NSCQ-MSX0853)。
关键词
重庆市
伦敦沙门氏菌
全基因组测序
分型溯源
耐药
Chongqing City
Salmonella London
whole-genome sequencing
classification and traceability
antibiotic resistance
