摘要
为研究5-氮胞苷(5-azacytidine, 5-AzaC)在水曲柳体细胞胚胎发生中的表观遗传调控作用,通过在培养基中添加20.0μmol·L-15-AzaC诱导水曲柳体细胞胚胎发生,以未添加5-AzaC为对照,采用甲基化敏感扩增多态性(methylation sensitive amplification polymorphism, MSAP)法分析外植体基因组DNA的胞嘧啶甲基化水平和模式,以及变异对体细胞胚胎发生的影响。结果表明:5-AzaC可以降低水曲柳外植体的褐化率,引起外植体基因组DNA甲基化状态发生变化,具体表现为全甲基化比例升高,半甲基化比例降低。5-AzaC抑制外植体基因组DNA的胞嘧啶甲基化,诱导基因组DNA去甲基化变异,从而降低体细胞胚胎的诱导率。研究结果表明,表观遗传参与调控树木体细胞胚胎发生,为树木体细胞胚胎发生提供了理论指导。
To investigate the role of 5-azacytidine(5-AzaC)in the epigenetic regulation of genomic DNA methylation during somatic embryogenesis in Fraxinus mandsharica Rupr.,somatic embryos were induced by adding 20.0μmol·L^(-1)5-azacytidine to the medium,with untreated samples as the control,methylation-sensitive amplification polymorphism(MSAP)was used to analyze the effects of cytosine methylation levels,patterns,and variations in the genomic DNA of explants on somatic embryogenesis.The results showed that,the browning rate of the explants was reduced by 5-AzaC treatment,which led to alterations in the genomic DNA methylation status,specifically an increase in fully methylated cytosines and a decrease in hemimethylated cytosines.5-AzaC inhibited cytosine methylation and induced DNA demethylation variation in explant genomic DNA,thereby reducing the induction rate of somatic embryos.These findings demonstrated that epigenetic mechanisms participate in regulating somatic embryogenesis in trees,which provided theoretical guidance for the study of tree somatic embryogenesis.
作者
丛建民
黄威剑
尹欣幸
刘金平
赵永
CONG Jianmin;HUANG Weijian;YIN Xinxing;LIU Jinping;ZHAO Yong(Crop Research Institute,School of Agricultural and Biological Engineering,Taizhou Vocational College of Science and Technology,Taizhou 318020,Zhejiang,China;College of Life Sciences,Baicheng Normal University,Baicheng 137000,Jilin,China)
出处
《浙江农业学报》
北大核心
2025年第8期1648-1657,共10页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(30872008)。
