摘要
为探究5-氮杂胞嘧啶(5-azacytidine,5-azaC)对陆地棉(Gossypium hirsutum L.)GhDMT7基因表达及种子萌发的影响,分析了5-azac处理下GhDMT7的表达水平和棉花种子生长性状。克隆了GhDMT7基因,通过生物信息学分析发现,GhDMT7蛋白为稳定的亲水性蛋白,无信号肽和跨膜结构。在盐胁迫条件下,棉花叶片中的丙二醛含量增加,表明膜损伤加剧。5-azaC对GhDMT7的表达呈剂量效应,低水平下轻微上调基因表达,高水平下则抑制表达,并降低5-甲基胞嘧啶(5mC)含量。此外,50μmol·L^(−1)的5-azaC处理显著提高了种子的发芽势和生长指标,对种子萌发起促进作用。以上研究结果为进一步研究GhDMT7在棉花生长发育中的功能奠定了基础,也为棉花耐盐新品种的培育提供了基因资源。
To investigate the effects of 5-azacytidine(5-azaC)on the expression of the GhDMT7 gene and seed germination of cotton(Gossypium hirsutum L.),this paper analyzed the expression level of GhDMT7 and the growth traits of cotton seed.The GhDMT7 gene was cloned and performed comprehensive bioinformatics analysis.The results showed that GhDMT7 protein was a stable hydrophilic protein with no signal peptide and transmembrane domains.Under salt stress conditions,the malondialdehyde content in cotton leaves increased,indicating exacerbated membrane damage.5-azaC exhibited a dosage effect on GhDMT7 expression with slightly up-regulated expression at low dosage and down-regulated expression at high dosage,and decreased 5-methylcytosine(5mC)content.Furthermore,the treatment with 50µmol·L^(-1)5-azaC significantly enhanced seed germination potential and growth parameters,which promoted seed germination.Above results laid a foundation for further studying the function of GhDMT7 on cotton growth and development,and provided genetic resources for breeding new varieties of saline-resistant cotton.
作者
杨志宁
陆许可
范亚朋
孙玉平
喻欣
王亮
高永健
古丽加依那什·哈不力
古丽沙西·拿斯依
吾木尔·阿不都
黄慧
张梦豪
王立东
陈筱
肖磊
张鑫蕊
王帅
陈修贵
王俊娟
郭丽雪
高文伟
叶武威
YANG Zhining;LU Xuke;FAN Yapeng;SUN Yuping;YU Xin;WANG Liang;GAO Yongjian;Gulijiayinashen Habuli;Gulishaxi Nasiyi;Wumuer Abudu;HUANG Hui;ZHANG Menghao;WANG Lidong;CHEN Xiao;XIAO Lei;ZHANG Xinrui;WANG Shuai;CHEN Xiugui;WANG Junjuan;GUO Lixue;GAO Wenwei;YE Wuwei(Cotton Engineering Research Center of Ministry of Education,College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;State Key Laboratory of Cotton Bio-breeding and Integrated Utilization,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Henan Anyang 455000,China;National Center of Technology Innovation for Comprehensive Utilization of Saline-alkali Land,Shandong Dongying 257000,China;Agricultural Technology Extension Center of Shawan City,Xinjiang Shawan 832100,China)
出处
《中国农业科技导报(中英文)》
北大核心
2025年第8期28-35,共8页
Journal of Agricultural Science and Technology
基金
国家棉花产业技术体系建设项目(CARS-15-02)
中国农业科学院科技创新工程项目。
