摘要
【目的】探究丛枝菌根真菌(AMF)对山黧豆耐盐性及β-ODAP含量的影响,为山黧豆在盐碱地中的推广应用提供理论依据。【方法】构建山黧豆毛状根与AMF的共培养体系,利用HPLC法分析NaCl胁迫条件下AMF共生对山黧豆毛状根中β-ODAP含量的调控作用,检测β-ODAP生物合成关键酶丝氨酸乙酰基转移酶(SAT)及β-腈基丙氨酸合成酶(CAS)活性。【结果】利用发根农杆菌C58C1浸染山黧豆幼苗诱导毛状根的产生。使用湿筛法获得AMF孢子,其在山黧豆毛状根中的接种可产生新生菌丝及新生孢子。醋酸墨水染色和巢式PCR分析表明,AMF在山黧豆毛状根中成功定殖。在盐胁迫条件下,50 mmol/L NaCl处理对于山黧豆毛状根的萌发和生长影响不显著,100 mmol/L和150 mmol/L NaCl处理显著降低毛状根的诱导率、根长和发根数目。AMF共生则显著促进NaCl胁迫条件下山黧豆毛状根的诱导率。此外,AMF与毛状根共生上调山黧豆β-ODAP生物合成关键酶SAT活性、下调CAS活性,减缓NaCl胁迫条件下山黧豆毛状根中β-ODAP的进一步积累。【结论】AMF共生显著改善山黧豆毛状根在NaCl胁迫条件下的生长状况,增强山黧豆毛状根的耐盐性。
[Objective]In the study,the effects of grass pea(Lathyrus sativus)hariy roots colonization by arbuscular mycorrhizal fungi(AMF)on its salt tolerance andβ-ODAP content were investigated to provide a theoretical basis for the improvement of L.sativus utilization in saline-alkali soil.[Method]A co-culture system of L.satiwus hairy roots and AMF were constructed.The regulatory role of AMF symbiosis onβ-ODAP content in the hairy roots of L.sativus under NaCl stress was analyzed by HPLC method,and the activities of the key enzymes inβ-ODAP biosynthesis including serine acetyltransferase(SAT)andβ-cyanoalanine synthase(CAS)were detected,separately.[Result]Hairy roots of L.satius was inducued via Agrobacterium rhizogenes C58Cl.AMF spores were isolated by wet sieving and sucrose gradient centrifugation.When it was inoculated to hairy roots of L.satius,novel hypha and spores were generated.The results of staining with ink and vinegar and nested PCR analysis suggested that the L.sativus hairy roots was colonized by AMF.Under the salt stress treatment,50 mmol/L NaCI stress had no significant effect on the germination and growth of L.satius hairy roots,while 100 mmol/L and 150 mmol/L NaCl significantly inhibited the germination and growth of L.sativus hairy roots.When L.sativus hairy roots were colonized by AMF under NaCl stress,the germination of hairy roots was greatly improved.Moreover,AMF symbiosis alleviated the accumulation ofβ-ODAP in the hairy roots of L satius under NaCl stress by up-regulating the activity of SAT and down-regulating the activity of CAS.[Conclusion]AMF symbiosis significantly improves the growth status of L.sativus hairy roots under Nacl stress and enhances the salt tolerance of L.sativus hairy roots.
作者
刘逸凡
张雪萍
郭慧莹
尚天雪
曹宁
刘晓宁
连荣芳
徐全乐
LIU Yi-fan;ZHANG Xue-ping;GUO Hui-ying;SHANG Tian-xue;CAO Ning;LIU Xiao-ning;LIAN Rong-fang;XU Quan-le(College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China;Dingxi Academy of Agricultrual Sciences,Dingxi,Gansu 743000,China;Medical School,Huanghe S&T University,Zhengzhou 450006,China)
出处
《西南农业学报》
北大核心
2025年第6期1162-1168,共7页
Southwest China Journal of Agricultural Sciences
基金
陕西省自然科学基金项目(2023-JC-YB-152)
河南省教育厅高等学校重点科研项目(25B180013)
国家食用豆产业技术体系(CARS-08-Z21)
河南省科技攻关项目(252102110271)
郑州市基础研究项目(0202240045)。
