摘要
选取青贮玉米为试验材料,设置3个多菌灵浓度和3个生育期处理,比较分析不同浓度的多菌灵对不同生育期青贮玉米生理特性和细菌群落的影响。结果表明:与乳熟期相比,大喇叭口期和蜡熟期的净光合速率和蒸腾速率分别下降了9.50mmol/(m^(2)·s)和15.20μmol/(m^(2)·s)、1.75mmol/(m^(2)·s)和2.59mmol/(m^(2)·s);乳熟期的保水性比大喇叭口期和蜡熟期分别提高了24.50%和13.20%;与大喇叭口期相比,乳熟期和蜡熟期的好氧细菌、大肠杆菌和乳酸菌数量都显著降低。多菌灵浓度对青贮玉米叶片的生理特性和细菌数量影响不显著。在属水平上,相对丰度最高的4种细菌分别是Sphingomonas、Massilia、Rhodococcus和Frigoribacterium。不同多菌灵浓度对假单胞菌属(Pseudonocardia)、芽孢杆菌属(Clostridium_sensu_stricto_1)、弗莱德门菌属(Friedmanniella)、未分类的蓝藻菌属(unclassified_o_Micrococcales)、藤黄单胞菌属(Luteimonas)、类固醇杆菌属(Steroidobacter)和不动杆菌属(Acinetobacter)的相对丰度具有显著影响。基于青贮玉米的利用方式和避免耐药性细菌的增加,本研究推荐在青贮玉米上施用低浓度的多菌灵。
This study aimed to reveal the effects of three carbendazim concentrations on physiological characteristics and bacterial community of silage maize in three maturity stages(big trumpet stage,milk stage,and dough stage).Results showed that the net photosynthetic and transpiration rate at the big trum-pet stage and dough stage decreased by 9.50µmol/(m^(2)·s)and 15.2µmol/(m^(2)·s),1.75 mmol/(m^(2)·s)and 2.59 mmol/(m^(2)·s),respectively(P<0.05)compared with milk stage.Compared with at the big trumpet stage and dough stage,the moisture retention capacity at the milk stage increased by 24.50%and 13.20%,respectively(P<0.05).The numbers of aerobic bacteria,Escherichia coli,and lactic acid bacte-ria at the milk stage and dough stage were significantly reduced(P<0.05)compared with the big trumpet stage.The concentration of carbendazim had no significant impact on the physiological characteristics or bacterial numbers of silage maize.At the genus level,the four bacteria with the highest relative abundance were Sphingomonas,Massilia,Rhodococcus,and Frigoribacterium.Different concentrations of carben-dazim significantly affected the relative abundances of Pseudonocardia,Clostridium_sensu_stricto_1,Friedman-niella,unclassified_o_Micrococcales,Luteimonas,Steroidobacter,and Acinetobacter(P<0.05).Consider-ing the utilization of maize silage and the need to prevent the proliferation of drug-resistant bacteria,this study recommends applying low concentration carbendazim on silage maize.
作者
唐国建
杨秋
徐庆标
杨金梅
蒙元燕
张小龙
许留兴
TANG Guojian;YANG Qiu;XU Qingbiao;YANG Jinmei;MENG Yuanyan;ZHANG Xiaolong;XU Liuxing(School of Biological Science and Technology,Liupanshui Normal University,Liupanshui 553004,China;School of Agriculture and Life Sciences,Zhaotong University,Zhaotong 657000,China)
出处
《中国草地学报》
北大核心
2025年第8期96-105,共10页
Chinese Journal of Grassland
基金
六盘水市科技创新平台建设项目(52020-2024-PT-2)
云南省基础研究计划青年项目(202201AU070161)
云南省地方本科高校基础研究联合专项(202301BA070001-120)
贵州省科技厅基础研究计划(自然科学)青年引导项目(黔科合基础QN[2025]265)。
关键词
青贮玉米
多菌灵
生育期
生理特性
细菌群落
Silage maize
Carbendazim
Maturity stage
Physiological characteristic
Bacterial com-munity
