摘要
为了攻克利用DNA指纹技术进行玉米种子纯度鉴定中存在的技术难关,对玉米单株幼芽DNA快速提取 方法进行了研究,结果表明:利用新方法提取DNA,不用液氮、不用研磨、不用离心、不用沉淀,不用SDS、不用CTAB、 不用氯仿,并且提取的DNA不降解、完全可以满足利用SSR分子标记进行DNA指纹分析的需要。一个人提取100 株幼芽(1个检测样品)DNA只需30 min左右,1 d可以提取2000株幼芽(20个检测样品)的DNA,为DNA指纹技术 在玉米种子纯度及真伪快速鉴定中的广泛应用解决了关键性技术难题。
In order to resolve the critical problem in maize purity identification using.DNA fingerprint, a new rapid DNA extraction method was developed for maize single seedling. This method would not need liquid nitrogen, mechanical grinding, centrifugation, no precipitation, SDS, CTAB, nor chloroform. The quality of DNA extracted using this method was suitable for SSR analysis. It cost about 30 minutes to extract DNA from each of 100 seedlings, and in a single day, one person can complete DNA isolation from more than 2 000 individuals. It resolved the critical technique problem of DNA fingerprint widely used in identification of maize seed purity.
出处
《华北农学报》
CSCD
北大核心
2005年第1期38-40,共3页
Acta Agriculturae Boreali-Sinica
基金
北京市科技新星项目
