摘要
籽粒百粒重是大豆产量构成三要素之一,百粒重大小决定大豆产量并影响其商品性,百粒重也是大豆遗传改良关键性状。为进一步挖掘籽粒大小相关基因,解析籽粒大小的调控机制。利用碳离子束(Carbon Ion Beam,CIB)辐射野生大豆(ZYD7068),构建了200份百粒重变异丰富的野生大豆突变群体。结果表明,群体籽粒百粒重在4.37~16.74 g之间,平均值为7.13 g,突变群体百粒重较野生大豆百粒重提高了4.98倍。对200个突变体采用全基因组测序,共获得184.1亿bp的测序数据,基因组覆盖率68.10%~70.96%。突变主要是单碱基突变(SNP),其中C到T突变占突变总数的40%。对候选SNP基因进行富集分析,突变基因主要富集在Go:0009626(植物超敏反应)、Go:0034050(共生体诱导宿主程序性细胞死亡)、Go:0012501(程序性细胞死亡)、Go:0043680(丝状器)以及Go:0045087(先天免疫反应)等途径。利用全基因组测序获得的SNPs标记,采用MLM模型,对突变群体籽粒百粒重进行全基因组关联分析(Genome-Wide Association GWAS),共检测到71个关联SNPs(-lgP>11),分别位于大豆2、3、5、6、9、10、14、16、18号染色体上,其中5、10号染色体和14号染色体以及18号染色体分别检测到9、21、9、23个SNPs,71个关联SNPs位点涉及33个基因。
The 100-grain weight is one of the three elements that constitute the yield of soybeans(Glycine max).The weight per 100-grain determines the yield of soybeans and affects their commercial quality.The 100-grain weight is also a key trait for genetic improvement of soybeans.To further explore the genes related to grain size and analyze the regulatory mechanism of grain size.By radiating wild soybeans(ZYD7068)with a Carbon Ion Beam(CIB),a mutant population of 200 wild soybeans with rich 100-grain weight variations was constructed.The results showed that the 100-grain weight of the population grains ranged from 4.37 g to 16.74 g,with an average of 7.13 g.The 100-grain weight of the mutant population was 4.98 times higher than that of wild soybeans.Whole-genome sequencing was performed on 200 mutants,obtaining a total of 18.41 billion bp of sequencing data,with genomic coverage ranging from 68.10%to 70.96%.Mutations were mainly single-base mutations(SNP),among which C to T mutations account for 40%of the total number of mutations.Enrichment analysis of candidate SNP genes was conducted,and the mutant genes were mainly enriched in pathways such as Go:0009626(plant hypersensitivity reaction),Go:0034050(symbiont-induced host programmed cell death),Go:0012501(programmed cell death),Go:0043680(filamentous organs),and Go:0045087(innate immune response).Using SNP markers obtained from whole-genome sequencing and the MLM model,a genome-wide association was conducted on the 100-grain weight of the mutant population.A total of 71 associated SNPs were detected(-lg P>11).They were respectively located on chromosomes 2,3,5,6,9,10,14,16 and 18 of soybeans.Among them,9,21,9 and 23 SNPs were respectively detected on chromosomes 5,10,14 and 18.The 71 associated SNPs loci involved 33 genes.
作者
刘秀林
赵克臻
王雪扬
张丰屹
袁荣强
张春蕾
任洪雷
苗丽丽
王家军
张必弦
LIU Xiulin;ZHAO Kezhen;WANG Xueyang;ZHANG Fengyi;YUAN Rongqiang;ZHANG Chunlei;REN Honglei;MIAO Lili;WANG Jiajun;ZHANG Bixian(Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Heilongjiang Academy of Black Soil Conservation and Utilization,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)
出处
《种子》
北大核心
2025年第7期1-10,48,共11页
Seed
基金
黑龙江省重点研发计划(JD2023SJ28)
黑龙江省创新跨越工程(CX23TS04、CX23ZD04)
黑龙江省科研院所科研业务费项目(CZKYF2023-1-A001)
“十四五”重点研发计划项目(2022YFD1200704-2)。
关键词
大豆
百粒重
GWAS
Glycine max
100-grain weight
genome-wide association analysis
