摘要
【目的】探究抗裂荚分子机制,挖掘抗裂荚大豆种质资源,可为揭示大豆裂荚性遗传和驯化机理、加速南方大豆抗裂荚新品种的选育提供依据。【方法】结合全基因组关联分析定位和野生栽培大豆群体遗传多样性分析,探寻大豆裂荚性相关基因。使用302份大豆材料进行2年表型测定,借助95744个单核苷酸多态性标记进行全基因组关联分析。使用包括1308份栽培大豆和203份野生大豆的重测序数据进行QTL区段序列的遗传多样性分析。【结果】通过全基因组关联分析检测到3个表型变异解释率大于10%的QTL位点,分别为qPdh-Chr08(Gm08:3048312)、qPdh-Chr15(Gm15:312814)和qPdh-Chr16(Gm16:29951529)。其中qPdh-Chr16是已知基因pdh1(Pod dehiscence habit 1)。结合野生和栽培大豆重测序数据发现,qPdh-Chr08和qPdh-Chr15内存在人工选择导致的群体分化序列,发现2个重要候选基因Glyma.08G038600和Glyma.15G003600。根据功能注释发现,这两个基因参与生长素和木质素的代谢。【结论】使用2年数据检测到大豆裂荚性2个新的QTL位点qPdh-Chr08和qPdh-Chr15,并在QTL区域内鉴定野生栽培大豆分化基因,最终筛选到2个重要候选基因。
【Objective】Investigating molecular mechanisms and germplasm resources of pod dehiscence resistance would provide evidence for uncovering the genetic and domestication mechanism of pod dehiscence resistance in soybean as well as accelerating the breeding of new soybean varieties with pod dehiscence resistance on southern China.【Method】With genome-wide association studies(GWAS)and genetic diversity analysis between wild and cultivated soybean populations,the genes related to soybean pod dehiscence habit were explored.The phenotype determination of 302 soybean varieties were conducted,and GWAS was performed using 95744 single nucleotide polymorphism markers in 2 years.And analysis of genetic diversity in QTL segment was carried,with the resequencing data of 1308 cultivated soybeans and 203 wild soybeans.【Result】Through GWAS,three QTL loci that explained greater than 10%of the observed phenotypic variance were detected,namely qPdh-Chr08(Gm08:3048312),qPdh-Chr15(Gm15:312814),and qPdh-Chr16(Gm16:29951529).Among them,qPdh-Chr16 was known gene pdh1(Pod dehiscence habit 1).Combining the resequencing data of wild and cultivated soybeans,it was found that there were population differentiation sequences caused by artificial selection in regions of qPdh-Chr08 and qPdh-Chr15.And two important candidate genes Glyma.08G038600 and Glyma.15G003600 were identified based on population differentiation sequences analysis.Through functional annotation,it was found that these two genes were involved in the metabolism of auxin and lignin,respectively.【Conclusion】Two new QTL loci qPdh-Chr08 and qPdh-Chr15 of soybean pod dehiscence habit were detected using two-year’s data.Differential genes between wild and cultivated soybean are identified within the QTL region,and two important candidate genes are ultimately identified.
作者
宋英培
王灿
周会汶
孔可可
许孟歌
王瑞凯
SONG Ying-pei;WANG Can;ZHOU Hui-wen;KONG Ke-ke;XU Meng-ge;WANG Rui-kai(College of Pharmacy and Life Science,Jiujiang University,Institute of Jiangxi Oil-Tea Camellia/Soybean Research Institute,Jiujiang 332005)
出处
《生物技术通报》
北大核心
2025年第2期97-106,共10页
Biotechnology Bulletin
基金
国家自然科学基金项目(32260484,31760392)
江西省自然科学基金项目(20232BAB215028,20224BAB215014)
江西省现代农业产业技术体系建设专项(JXARS-24-01)。
关键词
大豆
裂荚性
全基因组关联分析
遗传多样性
作物驯化
soybeans
pod dehiscence habit
whole genome association analysis
genetic diversity
crop domestication
