摘要
为阐明脑樟型樟树叶片药效物质基础,本研究采用Sephadex LH-20凝胶柱色谱和硅胶柱色谱等方法,对其叶片95%乙醇提取物的乙酸乙酯部位进行分离纯化,并通过NMR、MS等方法鉴定化合物的结构;此外,采用带毒平板法和菌丝生长速率法测定上述化合物对油茶炭疽病病菌菌丝生长的抑制活性。从脑樟型樟树叶片乙醇提取部位的乙酸乙酯萃取物中得到10个化合物,分别为水仙苷(1)、阿亚黄素(2)、鸦胆子苦素D(3)、角鲨烯(4)、橄榄苦苷(5)、β-胡萝卜素(6)、长春碱(7)、α-红没药醇(8)、异阿魏酸(9)、7-甲基黄嘌呤(10)。其中化合物2、3、5、7、9、10均首次从脑樟型樟树中分离得到。活性筛选结果显示,在浓度为30 mg/mL时,化合物9、7和2对该菌丝生长的抑制率依次为62.73%±2.73%、54.55%±1.82%和30.91%±5.46%。
To elucidate the material basis of the pharmacological effects of leaves of camphor-type Cinnamomum camphora,the study was carried out to isolate and purify the chemical components from the ethyl acetate site of 95%ethanol extract of the plant by Sephadex LH20 gel and silica gel column chromatography and identify the structures of the compounds by modern spectroscopic methods such as NMR,MS,and so on.Furthermore,the inhibitory activities of the above compounds against the mycelial growth of pathogen of anthracnose in C.oleifera were determined by virus-bearing plate method and mycelial growth rate method.Ten compounds were obtained from the ethyl acetate extract of the ethanol extract of the leaves of camphor-type C.camphora,which were narcissoside(1),ayanin(2),bruceine D(3),squalene(4),oleuropein(5),β-carotene(6),vinblastine(7),α-bisabolol(8),isoferulic acid(9)and 7-methylxanthine(10).Among them,compounds 2,3,5,7,9 and 10 were isolated from leaves of camphor-type C.camphora for the first time.The results of activity screening showed that the inhibition rates of compounds 9,7 and 2 on the mycelial growth were 62.73%±2.73%,54.55%±1.82%and 30.91%±5.46%at the concentration of 30 mg/mL.
作者
俞静怡
张学斌
周升团
胡文杰
YU Jing-yi;ZHANG Xue-bin;ZHOU Sheng-tuan;HU Wen-jie(School of Life Sciences,Jinggangshan University,Ji′an 343009,China)
出处
《天然产物研究与开发》
北大核心
2025年第8期1471-1477,共7页
Natural Product Research and Development
基金
江西省自然科学基金面上项目(20232BAB205044,20181BAB204014)
江西省教育厅科学技术研究重点项目(GJJ2201609)。
关键词
樟树
脑樟型
分离鉴定
化学成分
抗菌活性
Cinnamomum camphora
camphor-type
isolation and identification
chemical compound
antifungal activity
