摘要
采用超声破碎法和甲醇:二氯乙烷=2:1(v/v)溶剂来萃取小球藻中类胡萝卜素类化合物。分别采用了3种不同的色谱分离方法,最后确定小球藻中类胡萝卜素类化合物的最优色谱分离条件为甲醇(A):乙氰(B)=90:10(v/v),柱温为室温,流速1ml/min。用ZOBAXSB-C18色谱柱和ZOBAX Eclipse plus C18色谱柱进行分离试验时发现,ZOBAX Eclipse plus C18色谱柱的分离效果良好。最后确定小球藻中叶黄素含量为2.312mg/g。
Using ultrasonic breaker,menstruum methanol：2-chlorine methane（2：1） to extract the carotenoids of Chlorella sp.3 methode have been compared to get the best mobile phase.Mixture methanol（A）;acetonitrile（B）=90：10 have been proved to be the best one.Carotenoids were eluted and monitored at 450 nm,velocity of flow is 1ml/min at room temperature.Disassociation result show ZOBAX Eclipse plus C18 column is the best one when compared with ZOBAX SB-C18 column,and content of lutein in Carotenoids is 2.312mg/g.
出处
《现代生物医学进展》
CAS
2010年第8期1546-1549,共4页
Progress in Modern Biomedicine
基金
湖南省教育厅资助项目(08C762)
