摘要
阳离子质子转运体(cation/H+exchanger,CHX)基因家族成员参与调控植物体内质子和阳离子的转运,并在植物生长发育和非生物胁迫应答中发挥重要作用。为解析拟南芥AtCHX19基因在盐碱胁迫应答中的功能,对拟南芥28个AtCHX基因家族成员进行分析。结果表明,拟南芥AtCHX基因可分为5个亚家族,其基因结构和蛋白基序十分保守。组织表达和非生物胁迫表达分析发现,AtCHX19在拟南芥根中表达且响应盐碱胁迫。进一步对拟南芥突变体进行耐盐碱分析表明,AtCHX19基因缺失降低了突变体对高盐和苏打盐碱胁迫的耐性,且突变体中的Na+含量显著高于野生型。以上研究结果初步明确了AtCHX19基因通过调控Na+转运响应盐碱胁迫应答,为进一步阐明CHX家族基因介导的耐逆分子机制和信号传导通路提供了数据支持。
Cation/H+exchanger(CHX)gene family participate in the regulation of plants proton and cation transport,and play an important role in plant growth and development and abiotic stress response.To analyze the Arabidopsis AtCHX genes in response to salt-alkali stress,28 AtCHX genes of Arabidopsis were analyzed.The results showed that AtCHX genes were divided into 5 subfamilies,and their structures and protein sequence motifs were very conserved.Tissue expression and abiotic stress expression analysis found that AtCHX19 expressed in roots of Arabidopsis and up-regulated by salt-alkali stress.Further salt-alkali tolerance analysis of Arabidopsis mutant showed that the deletion of AtCHX19 gene reduced tolerance to high salt and soda saline stress,and Na+content of mutant was significantly higher than that of wild type.Above results initially clarified that the AtCHX19 gene responded to saline-alkali stress by regulating Na+transport,which provided data support for further elucidating the stress resistant molecular mechanism and signaling transduction pathway mediating by CHX.
作者
关永旭
孙志成
王研
李媛
孙晓丽
贾博为
孙明哲
GUAN Yongxu;SUN Zhicheng;WANG Yan;LI Yuan;SUN Xiaoli;JIA Bowei;SUN Mingzhe(Crop Stress Molecular Biology Laboratory,College of Agriculture,Heilongjiang Bayi Agriculture University,Heilongjiang Daqing 163319,China)
出处
《中国农业科技导报(中英文)》
北大核心
2025年第8期60-72,共13页
Journal of Agricultural Science and Technology
基金
国家自然科学基金项目(32101672,32341033)
黑龙江省自然科学基金项目(YQ2023C035)
黑龙江省“双一流”学科协同创新成果项目(LJGXCG2023-072)
黑龙江八一农垦大学引进人才科研启动项目(XYB202203)
黑龙江八一农垦大学“三纵”青创人才(自然)项目(ZRCQC202302)
黑龙江八一农垦大学研究生创新科研项目(NXYYJSCX2022-Y13)
黑龙江八一农垦大学大学生创新创业训练计划项目(202310223159)。
