摘要
以沙棘(Hippophae rhamnoides)品种深秋红为试验材料,克隆Na^(+)/H^(+)逆向转运蛋白(NHX)家族成员中NHX1蛋白的基因,并对沙棘NHX1蛋白(HrNHX1)进行生物信息学分析。结果表明,HrNHX1基因全长约1 600 bp,编码一个由538个氨基酸残基组成的酸性疏水蛋白。该蛋白的分子式为C_(2754)H_(4269)N_(672)O_(759)S_(21),总原子数为8 502,相对分子质量为59 637.71。HrNHX1属于稳定蛋白,含有13个跨膜区,不具有信号肽,为非分泌蛋白,且包含1个NHX蛋白家族特有的Na^(+)/H^(+)exchanger结构域。亚细胞定位分析显示HrNHX1蛋白定位在液泡膜上。同源比对分析表明,HrNHX1蛋白序列与雷蒙德氏棉(Gossypium raimondii)、陆地棉(Gossypium hirsutum)、川桑(Morus notabilis)、橡胶树(Hevea brasiliensis)NHX1蛋白序列具有较高的序列相似性。系统进化树分析发现,HrNHX1蛋白序列与水稻、小麦具有抗旱、耐盐功能的NHX蛋白序列聚类于同一进化分支,表明它们在进化上具有较高的亲缘关系。HrNHX1基因在增强植物抗旱性和耐盐性方面发挥重要作用,通过调控其编码HrNHX1蛋白的活性或该基因的表达水平,可有效提升植物对干旱和盐胁迫的适应能力。
Using Shenqiuhong Hippophae rhamnoides as the experimental material,the gene encoding the NHX1 protein of the Na^(+)/H^(+)antiporter(NHX)family was cloned,and bioinformatic analysis was performed on the Hippophae rhamnoides NHX1 protein(HrNHX1).The results indicated that the full length of the HrNHX1 gene was approximately 1600 bp,encoding an acidic hydrophobic protein consisting of 538 amino acid residues.The molecular formula of this protein was C_(2754)H_(4269)N_(672)O_(759)S_(21),with a total of 8502 atoms and a relative molecular mass of 59637.71.HrNHX1 belonged to a stable protein,containing 13 transmembrane domains,lacking a signal peptide,being a non-secretory protein,and containing one Na^(+)/H^(+)exchanger domain characteristic of the NHX protein family.Subcellular localization analysis showed that the HrNHX1 protein was localized on the vacuolar membrane.Homology alignment analysis indicated that the HrNHX1 protein sequence had high sequence similarity with the NHX1 protein sequences of Gossypium raimondii,Gossypium hirsutum,Morus notabilis,and Hevea brasiliensis.Phylogenetic tree analysis found that the HrNHX1 protein sequence clustered on the same evolutionary branch with the NHX protein sequences of rice and wheat,which had drought resistance and salt tolerance functions,suggesting they had a close evolutionary relationship.The HrNHX1 gene played an important role in enhancing plant drought resistance and salt tolerance,and regulating the activity of its encoded HrNHX1 protein or the expression level of this gene could effectively improve plant adaptability to drought and salt stress.
作者
崔长珍
霍彦波
张思宇
柴乖强
鲍亮亮
段义忠
CUI Chang-zhen;HUO Yan-bo;ZHANG Si-yu;CHAI Guai-qiang;BAO Liang-liang;DUAN Yi-zhong(College of Advanced Agricultural Sciences,Yulin University,Yulin 719000,Shaanxi,China;Yulin Genuine Qin Medicinal Materials Research Institute,Yulin University,Yulin 719000,Shaanxi,China)
出处
《湖北农业科学》
2025年第7期192-197,共6页
Hubei Agricultural Sciences
基金
陕西省榆林市中青年科技领军人才基金资助项目(2023KJZG03)
陕西省教育厅产业化培育基金资助项目(22JC065)
陕西省榆林市产学研基金资助项目(CXY202181)
陕西省榆林道地秦药研究院基金资助项目(2021hx36)
横向基金资助项目(H2023060236)。
