摘要
R2R3-MYB转录因子是植物中最大的MYB亚家族,广泛参与调节植物生长发育和胁迫响应。对板栗R2R3-MYB基因家族进行全基因组鉴定,分析其在染色体上分布、亚细胞定位、系统进化关系、蛋白基序、基因结构、保守结构域,最后通过转录组和RT-qPCR分析CmR2R3-MYB基因在干旱胁迫下的表达。结果表明,共鉴定出141个CmR2R3-MYB基因,不均等地分布于12条染色体与1条contig片段。系统进化树将其分为6个亚家族。有7个CmR2R3-MYB基因在干旱胁迫下差异表达,其中CmMYB89表达量上调,CmMYB32、CmMYB95、CmMYB91、CmMYB119、CmMYB131及CmMYB104表达量下调,表明这些基因可能与板栗抗旱性密切相关。以上研究结果为进一步探究R2R3-MYB基因家族在板栗响应干旱胁迫方面的作用奠定了理论基础。
R2R3-MYB transcription factors are the largest MYB subfamily in plants and are widely involved in regulating plant growth and development and stress response.R2R3-MYB gene family of Chinese chestnut was genome-widely identified,and the chromosome distribution of these genes,subcellular localization prediction,phylogenetic relationships,protein motif,gene structure and sequence alignment of conserved domains were analyzed.Finally,the expression of the CmR2R3-MYB gene family under drought stress was analyzed by transcriptome and RT-qPCR.The results showed that total 141 CmR2R3-MYB genes were unequally distributed on 12 chromosomes and 1 contig fragment.And the phylogenetic tree divided them into 6 groups.There were 7 CmR2R3-MYB genes differentially expressed under drought stress.Among them,CmMYB89 was upregulated,while CmMYB32,CmMYB95,CmMYB91,CmMYB119,CmMYB131 and CmMYB104 were downregulated,which indicated that these 7 genes could be involved in drought resistance of Chinese chestnut.Above results provided theoretical foundations for further exploring the role of the R2R3-MYB gene family in response to drought stress of Chinese chestnut.
作者
刘亚明
杨涌
康肖肖
王萌
王东升
张海娥
LIU Yaming;YANG Yong;KANG Xiaoxiao;WANG Meng;WANG Dongsheng;ZHANG Haie(Hebei Province Chestnut Industry Collaborative Innovation Center,Engineering Research Center of Chinese Chestnut Industry Technology of Ministry of Education,College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Hebei Qinhuangdao 066004,China)
出处
《中国农业科技导报(中英文)》
北大核心
2025年第8期47-59,共13页
Journal of Agricultural Science and Technology
基金
国家重点研发计划项目(2022YFD2200400)
河北省重点研发计划项目(21326304D)
河北科技师范学院博士启动基金项目(2023YB026)。
