摘要
目的 建立同时测定菊花决明子茶中8种有效成分(绿原酸、咖啡酸、阿魏酸、毛蕊花糖苷、木犀草苷、芦丁、槲皮素及橙黄决明素)的高效液相色谱(high performance liquid chromatography,HPLC)分析方法。方法 采用Waters HSS T3 C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸溶液为流动相进行梯度洗脱(0~20 min:10%~30%甲醇;20~30 min:30%~60%甲醇;30~40 min:60%~10%甲醇;40~50 min:10%甲醇),流速1.0 mL/min,柱温30℃,检测波长320 nm。结果 8种成分在对应的浓度区间内呈现出良好的线性关系(r≥0.9968),平均加样回收率范围为87.02%~101.95%,相对标准偏差0.65%~1.39%。结论 该方法准确可靠,适用于菊花决明子茶的质量控制,分析结果显示不同批次产品中抗氧化成分含量存在显著差异。
Objective To establish an high performance liquid chromatography(HPLC)method for the simultaneous determination of 8 kinds of active components(chlorogenic acid,caffeic acid,ferulic acid,verbascoside,luteoloside,rutin,quercetin and aurantio-obtusin)in chrysanthemum and cassia seed tea.Methods Separation was performed on a Waters HSS T3 C18 column(250 mm×4.6 mm,5μm)using a gradient elution of methanol-0.1%phosphoric acid solution(0–20 min:10%–30%methanol;20–30 min:30%–60%methanol;30–40 min:60%–10%methanol;40–50 min:10%methanol)at a flow rate of 1.0 mL/min.The column temperature was maintained at 30℃with detection wavelength set at 320 nm.Results The 8 kinds of components showed good linear relationships within their respective concentration ranges(r≥0.9968).The average recoveries ranged from 87.02%to 101.95%with relative standard deviations of 0.65%–1.39%.Conclusion The developed method is accurate and reliable for quality control of chrysanthemum and cassia seed tea,revealing significant variations in antioxidant component content among different batches.
作者
庞文娟
秦海钰
董斌
孙东德
陈荣红
PANG Wen-Juan;QIN Hai-Yu;DONG Bin;SUN Dong-De;CHEN Rong-Hong(Wuwei Drug Inspection and Testing Center,Wuwei 733000,China;Gansu Qilian Tibetan Medicine Co.,Ltd.,Wuwei 733000,China)
出处
《食品安全质量检测学报》
2025年第17期166-171,共6页
Journal of Food Safety and Quality
基金
武威市2024年度市级科技计划自筹经费项目(WW24B01GY005,WW24B01GY006,WW24B01NY052)
武威市2024年度市级科技计划项目(WW24A01QYJ006)。
关键词
菊花决明子茶
抗氧化成分
同时测定
高效液相色谱法
chrysanthemum and cassia seed tea
antioxidant components
simultaneous determination
high performance liquid chromatography
