摘要
为探究外源复合酶对厌氧共消化系统产甲烷效能的强化策略,采用玉米秸秆和餐厨垃圾为混合基质,通过批次试验和响应面法优化了外源复合酶的组合及浓度;木质素单因子试验筛选了复合酶组分和浓度范围;通过响应曲面法设计了20组以漆酶、内切-β-1,4-葡聚糖酶和木聚糖酶浓度为变量的试验,并以产甲烷促进率作为优化目标,根据试验数据成功建立了模型并进行了方差分析,结果表明响应面分析确定漆酶(1.5~4.0U/mL)、木聚糖酶(12~20U/mL)和内切-β-1,4-葡聚糖酶(50~85U/mL)的范围内可到达高产甲烷促进率的酶组合作为合适的复合酶组分.连续流试验验证复合酶的效能表明:投加复合酶促进了水解酶和乙酸型产甲烷菌的增殖,释放更高活性的木质素、纤维素酶和厌氧消化酶,进而促使厌氧共消化系统的甲烷产率提高19.5%.
To explore the enhancement strategies for the methane production efficiency of anaerobic co-digestion systems by exogenous compound enzymes,corn stover and food waste were adopted as the mixed substrates.Subsequently,the combination and concentration of exogenous compound enzymes were optimized through batch experiments and the response surface methodology.In the single-factor experiment on lignin,the component and concentration range of the compound enzymes were screened.Moreover,20groups of experiments with the concentrations of laccase,endo-β-1,4-glucanase and xylanase as variables were designed by means of the response surface methodology,and with the methane production promotion rate as the optimization objective.Based on the experimental data,a model was successfully established and variance analysis was carried out.The results showed that response surface analysis identified enzyme combinations within the ranges of laccase(1.5~4.0U/mL),xylanase(12~20U/mL)and endo-β-1,4-glucanase(50~85U/mL),which could achieve a high methane production promotion rate,was finally determined as the appropriate component of the compound enzyme.The continuous flow test validated the efficacy of the composite enzyme,indicating that the addition of the compound enzyme promoted the proliferation of hydrolases and aceticlastic methanogens,released lignin,cellulase and anaerobic digestion enzymes with higher activities,and thus increased the methane production rate of the anaerobic co-digestion system by 19.5%.
作者
常享琳
李治波
谢娟
曹思凡
李令虎
李玉友
王晓昌
陈荣
邢保山
CHANG Xiang-lin;LI Zhi-bo;XIE Juan;CAO Si-fan;LI Ling-hu;LI Yu-you;WANG Xiao-chang;CHEN Rong;XING Bao-shan(School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an710055,China;Department of Civil and Environmental Engineering,Tohoku University,Sendai 980-8579,Japan)
出处
《中国环境科学》
北大核心
2025年第8期4423-4433,共11页
China Environmental Science
基金
国家自然科学基金资助项目(52100162)
日本学术振兴会海外特别研究员项目(P24088)。
关键词
玉米秸秆
餐厨垃圾
复合酶
产甲烷
厌氧共消化
corn straw
food waste
compound enzyme
methane production
anaerobic co-digestion
