摘要
为研究酒糟沼气工程中互营丙酸氧化菌群,以不同阶段酒糟沼液为接种物,丙酸为唯一碳源,经过1年富集培养获得两个中温厌氧丙酸降解培养系JO-P和JN-P.丙酸在1.5-2个月转化为乙酸和甲烷.采用16S rRNA基因高通量测序技术,对培养系中细菌及古菌的群落结构进行解析.细菌解析结果表明,两个培养系中存在不同类型的丙酸降解菌:JO-P中存在3种丙酸降解相关功能微生物,与已培养丙酸降解菌Smithella propionica(16S rRNA基因相似性,99%)、Syntrophobacter wolinii(相似性95%)及Desulfobulbus propionicus(相似性100%)序列近缘,分别占细菌的37.61%、10.40%及1.34%.JN-P中存在1种与已培养丙酸降解菌Syntrophobacter wolinii近缘微生物(相似性95%),占细菌的24.90%;古菌解析结果表明,JO-P中氢营养型产甲烷菌Methanoculleus receptaculi(相似性100%)及乙酸营养型产甲烷菌Methanosaeta concilii(相似性100%)分别占95.09%和4.85%.JN-P中只存在氢营养型产甲烷菌Methanoculleus receptaculi(相似性100%),相对丰度99.98%.本研究表明Smithella propionica、Desulfobulbus propionicus及Syntrophobacter wolinii在酒糟沼气工程中降解丙酸发挥重要作用.
To investigate the propionate-degrading community of vinasse biogas plants,primary enrichments were made with propionate as the sole carbon source,using two stages of the vinasse biogas slurry as inoculum.Two stable mesophilic methanogenic propionate-degrading enrichments,JO-P and JN-P,were obtained after one year of successive transfers.Propionate was converted to methane and acetate within 1.5-2 months.The bacterial and archaeal community structures were investigated using 16S rRNA gene-based highthroughput sequencing.The results showed that different dominant propionate-degrading bacteria existed in the two enrichments.In the cultured JO-P,the predominant phylotypes were related to three cultured propionatedegrading species;Smithella propionica(99%similarity by 16S rRNA gene sequence),Syntrophobacter wolinii(95%similarity),and Desulfobulbus propionicus(100%similarity),which accounted for 37.61%,10.40%,and 1.34%of the total bacterial community,respectively.In cultured JN-P,phylotype related to Syntrophobacter wolinii(95%similarity)accounted for 24.9%of potential propionate-oxidizing bacterium.The archaeal community in the two cultures was also different.In cultured JO-P,hydrogenotrophic methanogen Methanoculleus receptaculi(100%similarity)and acetotrophic methanogen Methanosaeta concilii(100%similarity)accounted for 95.09%and 4.85%of the total archaeal community,respectively,while the hydrogenotrophic methanogen Methanoculleus receptaculi(100%similarity)was predominant in JN-P culture,accounting for 99.98%.This study revealed that Smithella propionica,Desulfobulbus propionicus,and Syntrophobacter wolinii play important roles in propionate degradation in vinasse biogas plants.
作者
谢彤彤
吴凯旋
迟明妹
孙晓娇
张培玉
于德爽
邱艳玲
XIE Tongtong;WU Kaixuan;CHI Mingmei;SUN Xiaojiao;ZHANG Peiyu;YU Deshuang;QIU Yanling(School of Environmental Science and Engineering,Qingdao University,Qingdao 266071,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第6期1406-1410,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(51878363)
山东省自然科学基金项目(ZR2019MEE028)资助。
