摘要
采用批式发酵方式,考察了黄瓜秸秆、番茄秸秆、茄子秸秆和辣椒秸秆分别与猪粪、牛粪和羊粪共消化产甲烷特性及关键微生物变化情况.结果表明:蔬菜秸秆与牲畜粪便共消化均发生了协同促进作用,且4种蔬菜秸秆与猪粪混合的产甲烷效果最好,其次为与牛粪混合,最后则是与羊粪混合.其中,黄瓜秸秆和茄子秸秆与猪粪配比为1:2时累积甲烷产量最大,分别为338.28和283.00mL/g VS,较各自秸秆单消化分别提高了65.15%和67.36%;番茄秸秆和辣椒秸秆与猪粪配比为1:4时最大,分别为317.90和251.17mL/gVS,较各自秸秆单消化分别提高了69.07%和92.93%.微生物分析结果显示,Syner-01在添加猪粪的样品和所有共消化样品中相对丰度较高;添加猪粪的共消化样品中产甲烷菌类群更丰富,甲烷囊菌属(Methanoculleus)和甲烷盘菌属(Methanoplanus)相对丰度更高,而添加牛粪后甲烷粒菌属(Methanocorpusculum)丰度更高,说明在蔬菜秸秆中添加不同粪便进行共消化时会引起氢营养型产甲烷菌的富集.
The characteristics of methane yields and the changes of key microorganisms in the co-digestion of cucumber straw,tomato straw,eggplant straw and pepper straw with pig manure,cow manure and sheep manure were investigated by batch fermentation.The co-digestion of vegetable straws with livestock manure had a synergistic effect,and the methanogenic effect of the four vegetable straws mixed with pig manure was the best,followed by cow manure and sheep manure.Among them,cucumber straw and eggplant straw with pig manure at a ratio of 1:2 had the maximum cumulative methane yields of 338.28mL/g VS and 283.00mL/g VS respectively,which increased by 65.15%and 67.36%compared to the single digestion of the respective straws.The maximum methane yields for tomato straw and pepper straw with pig manure were 317.90mL/g VS and 251.17mL/g VS at a ratio of 1:4,which were 69.07%and 92.93%higher than the single digestion of the tomato and pepper straws respectively.The microbial analysis showed that the relative abundance of Syner-01 was higher in the samples added with pig manure and in all co-digested samples.The methanogenic archaea taxa in the co-digested samples added with pig manure were more abundant,with higher relative abundance of Methanocellus and Methanoplanus,while the abundance of Methanocorpusculum were higher after adding cow manure,indicating that the addition of different manures to vegetable straws for co-digestion could lead to the enrichment of hydrotropic methanogenic archaea.
作者
孟艳
李屹
杜中平
韩睿
MENG Yan;LI Yi;DU Zhong-ping;HAN Rui(Qinghai Key Laboratory of Vegetable Genetics and Physiology,Academy of Agriculture and Forestry Sciences,Qinghai University,Qinghai Xining 810016,China)
出处
《中国环境科学》
CSCD
北大核心
2023年第S1期136-148,共13页
China Environmental Science
基金
青海省自然科学基金资助项目(2023-ZJ-928M)
国家科技部重点研发计划项目(2022YFD1602403)
关键词
蔬菜秸秆
共消化
甲烷
协同作用
微生物分析
vegetable straws
co-digestion
methane
synergies
microbial community analysis
