摘要
优化沙棘叶多酚(Hippophae rhamnoides leaf polyphenols,HRLP)的超声辅助提取工艺,并评价其抗氧化与降脂活性。研究超声时间、料液比、超声功率和超声温度4个因素对HRLP提取量的影响,在单因素试验的基础上采用Box-Behnken响应面法优化提取工艺参数,最优提取条件得到的HRLP进行体外实验来验证其抗氧化和降脂活性。结果表明:在超声时间42 min、温度61℃、功率410 W、料液比1∶47(g/mL)条件下多酚提取量最高,可达到18.07±0.74 mg/g。在此条件下得到的HRLP对ABTS、DPPH以及羟基自由基清除能力IC50值分别为0.11、0.25、0.54 mg/mL,对铁离子还原能力约为维生素C的1/6。降脂活性研究发现,HRLP最大油脂吸附量为38.7 mg/g,胆汁酸吸附率为62.45%,胰脂肪酶抑制活性IC50为0.85 mg/mL。HRLP提取工艺稳定可行,所得HRLP具有良好的抗氧化与降脂活性。该研究所建立的新方法操作简便易行,可实现HRLP的高效制备,为HRLP的工业化生产提供了新思路和新方法。
This study aimed to optimize the ultrasound-assisted extraction process of Hippophae rhamnoides leaf polyphenols(HRLP)and evaluate their antioxidant and lipid-lowering activities.The effects of four factors—ultrasonic time,solid-to-liquid ratio,ultrasonic power,and temperature—on HRLP yield were investigated.Based on single-factor experiments,the extraction parameters were optimized using the Box-Behnken response surface methodology.The HRLP obtained under optimal conditions was further subjected to in vitro experiments to validate its antioxidant and lipid-lowering activities.Results showed that the maximum polyphenol yield of 18.07±0.74 mg/g was achieved under the optimal conditions:ultrasonic time of 42 min,temperature of 61℃,power of 410 W,and solid-to-liquid ratio of 1∶47(g/mL).The HRLP exhibited IC 50 values of 0.11,0.25,and 0.54 mg/mL for scavenging ABTS,DPPH,and hydroxyl radicals,respectively,and its ferric ion reducing capacity was approximately 1/6 that of vitamin C.In lipid-lowering studies,HRLP demonstrated a maximum oil-binding capacity of 38.7 mg/g,a bile acid binding rate of 62.45%,and pancreatic lipase inhibitory activity with an IC 50 of 0.85 mg/mL.The extraction process proved stable and feasible,yielding HRLP with significant antioxidant and lipid-lowering properties.The novel method established in this study features simple operation and enables efficient preparation of HRLP,providing innovative strategies for its industrial-scale production.
作者
肖付才
邢晓轲
李颖奎
黄斐
XIAO Fu-cai;XING Xiao-ke;LI Ying-kui;HUANG Fei(College of Landscape and Food Engineering,Xuchang Vocational Technical College,Xuchang 461000,China)
出处
《天然产物研究与开发》
北大核心
2025年第7期1331-1340,共10页
Natural Product Research and Development
基金
河南省教育厅质量工程项目(2022-6)。
关键词
沙棘叶
多酚
超声提取
响应面优化
抗氧化活性
降脂活性
Hippophae rhamnoides leaf
polyphenols
ultrasound-assisted extraction
response surface optimization
antioxidant activity
lipid-lowering activity
