摘要
【目的】马口鱼(Opsariichthys bidens)是中国淡水养殖中极具开发潜力的经济鱼种,其肉质鲜美、适应性强,但人工繁育效率受制于亲鱼卵巢发育水平。本文旨在探究养殖密度对马口鱼亲鱼卵巢发育的影响。【方法】选取规格一致、体格健康的马口鱼亲鱼,随机分为4个密度梯度:低密度组(M_(1),2 kg/m^(3))、中密度组(M_(2),4 kg/m^(3))和2个高密度组(M_(3),6 kg/m^(3);M_(4),8 kg/m^(3))进行培育,试验周期为45 d,再进行卵巢切片观察与计数,计算性腺指数(GSI)、卵巢发育至Ⅳ时相期的比例(F);测定卵巢水分、粗蛋白质、粗脂肪、粗灰分、磷和钙等常规营养组分含量;统计催产率、孵化率、受精率。【结果】中密度组的性腺指数(13.35±1.97)%显著高于其他密度组(P<0.05);且中密度组卵巢发育至Ⅳ时相期的比例为(46.62±3.20)%,与其他组差异显著(P<0.05);在卵巢常规营养成分方面,与其他密度组相比,中密度组马口鱼卵巢水分含量显著降低(P<0.05),粗脂肪含量显著提高(P<0.05),而粗蛋白质、粗灰分、钙和磷的含量差异不显著(P>0.05);在繁殖力方面,中密度组的催产率(93.30±3.30)%和孵化率(81.40±4.00)%显著高于其他密度组(P<0.05),且受精率最优。【结论】上述结果表明,合理的养殖密度能在一定程度上提高马口鱼亲鱼卵巢发育水平。
[Background]Opsariichthys bidens is an economic fish species with great potential for development in freshwater aquaculture in China,with delicious meat and strong adaptability,but the efficiency of artifi-cial breeding is limited by the level of ovarian development of parental O.bidens.[Objective]The aim of the present study is to investigate the effect of stocking density on the ovarian development of parental O.bidens.[Methods]Parental O.bidens of the same specification and healthy physique were selected and randomly divided into four density gradients:a low density group(M1,2 kg/m^(3)),a medium density group(M2,4 kg/m^(3)),and two high density groups(M3,6 kg/m^(3) and M4,8 kg/m^(3))for cultivation,and the experimental cycle was 45 d.The ovarian sections of ovaries were observed and counted to calculate the gonadal index(GSI)and the proportion of phases of ovarian development to stageⅣ(F);the contents of conventional nutrient fractions such as moisture,crude protein,crude fat,crude ash,phosphorus and calcium of the ovaries were determined;and statistics analysis on the induced spawning rate,hatching rate and fertilization rate were carried out.[Results]The results showed that the gonadal index of the medium density group(13.35±1.97)%was significantly higher than those of the other density groups(P<0.05);and the phases of ovarian development to stageⅣin the medium density group was(46.62±3.20)%,which was significantly different from that of the other groups(P<0.05).Regarding the conventional nutrient composition of the ovaries,the moisture content of the ovaries of the O.bidens in the medium density group was significantly lower(P<0.05)and the crude fat content was signific-antly higher(P<0.05),while the differences in the contents of crude protein,crude ash,calcium,and phosphor-us were not significant(P>0.05).In terms of fecundity,the medium density group had significantly(P<0.05)higher induced spawning rate(93.30±3.30)%and hatching rate(81.40±4.00)%than those of the other density groups,and had the optimum fertilization rate.[Conclusion]The above results indicate that reasonable stocking density can improve the level of ovarian development of parental O.bidens to a certain extent.
作者
陈家乐
陈度煌
李文盛
薛凌展
吴妹英
吴斌
黄文树
CHEN Jiale;CHEN Duhuang;LI Wensheng;XUE Lingzhan;WU Meiying;WU Bin;HUANG Wenshu(Fisheries College,Jimei University,Xiamen 361021,China;Fujian Institute of Freshwater Aquatic Research,Fuzhou 350002,China)
出处
《渔业研究》
2025年第4期500-507,共8页
Journal of Fisheries Research
基金
福建省种业创新和产业化工程项目(2021FJSCZY04)
福建省属公益类科研院所基本科研专项(2022R1014004)。
